MOLECULAR IMMUNOHEMATOLOGY AND AUTOMOATION

Question 1

BC and plasma reacts in a
chamber with air bubble barrier to keep unbound
plasma from
neutralizing
antiglobulin reagentlocated in lower gel
column

Answer 1

GEL TECHNOLOGY/ COLUMN AGGLUTINATION
TECHNOLOGY

Question 2

RecentlyRecently transfused patients or BM transplant
recipients are expected to have

Answer 2

mixed
populations of RBC thereby causing MF
reaction transfused patients or BM transplant
recipients are expected to have mixed
populations of RBC thereby causing MF
reaction `

Question 3

range of rxn

Answer 3

bashain

Question 4

The gel acts as a
sieve, allowing smaller molecules to pass
through while trapping larger molecules
based on size.

Answer 4

Extran-Acrylamide Gel –

Question 5

Measured Volumes of Reagents in column agglutination technology

Answer 5

• • 1:2 (1 antibody/serum:2 rcs)
    25ul of plasma or serum and 50ul of
    0.8 RBCs

Question 6

Provides stable, well-defined endpoints of
agglutinaion reaction that can be observed for
3 days

Answer 6

column agglutination test

Question 7

causing stack like appearance which
will be perceived as agglutination so di
siya makakapsok sa gel

Answer 7

Rouleaux Formation

Question 8

grossly icteric samples =

Answer 8

might clog
column. There will be diffused
blotches of RBC which would need to
be clarified by centri an filtration

Question 9

test where substrate is used instead of indicator
cells

Answer 9

ELISA

Question 10

ANTIGEN POSITIVE VS ANTIGEN NEGATIVE RED CELL

Answer 10

● Antigen-Positive red cells from donor or
patient sources adhere to the sides and
bottom of the wells.
● Antigen-negative red cells from donor or
patient sources settle to the bottom of the
well and form a red cell button after
centrifugation

Question 11

EXPLAIN SANDWHICH TECHNIQUE

Question 12

Detects Class 1 HLA

Answer 12

MICROLYMPHOTOXICITY TEST

Question 13

● Complement-mediated cytotoxicity test

Answer 13

MICROLYMPHOTOXICITY TEST

Question 14

Separates rbc from wbc and plasma

Answer 14

● Ficoll-Hypaque

Question 15

SAMPLE FOR MICROLYMPHOTOXICITY TEXT

Answer 15

BUFFY COAT

Question 16

PROCESS OF MICROLYMPHOTOXICITY TEST

Answer 16

Lymphocytes + Anti-HLA (multiparous
women) + Complement (Rabbit Serum) +
Stain (Trypan Blue or Eosin Y)

Question 17

● Tests for Class II compatibility

Answer 17

MIXED LYMPHOCYTE CULTURE

Question 18

MIXED LYMPHOCYTES ACTING

Answer 18

Mitomycin C treated or irradiated donor
lymphocytes + Live cells of recipient or
patient

Question 19

Mitomycin C kills donor lymphocyte
but cell membrane which contains
antigens and components are still
intac

Answer 19

Mitomycin C

Question 20

○ Frozen Cells- Cells more susceptible
to lysis (False Positives) –
○ HLA Antigen frequencies vary among
different races

Answer 20

● Lymphocytotoxicity test

Question 21

In vitro replication of dna substances to have
detectable quantity

Answer 21

PCR

Question 22

Put tubes in high heat to break
hydrogen bonds that links dna

Answer 22

● DENATURATION (94-96C)

Question 23

Monitors real time
quantification
● In traditional METHOD need
pa electrophoresis

Answer 23

■ Q-PCR