Molecular Technologies

Question 1

DNA Cloning

Answer 1

process of isolating then making many copies of a particular DNA sequence using a plasmid vector

applications: probes for ID of similar or identical gene or RNA, expression of cloned gene in same or different organism

Question 2

DNA cloning steps

Answer 2

1. restriction enzyme cut
2. base paring of sticky ends produces various combinations
3. DNA ligase seals the strands

Question 3

Restriction enzymes

Answer 3

restriction endonucleases that recognize specific DNA sequences and cleave both strands of DNA
- different ones target different sequences

Question 4

PCR Technology

Answer 4

method for multiplying target DNA sequences for genetic analysis synthetically
- developed by Kary Mullis