Mono part 2 Flashcards
How is the serum sample collected for monospot?
With or without anticoagulant
How is the serum prepped?
Centrifuged amd serum should be clear and unhemolyzed
What is the test for mono?
Monospot latex test kit
Prior to testing mon what must be done?
- Centrifuge the specimen (serum or plasma) to sediment cellular elements
- Test the specimen within 24 hours of blood collection if stored between 2-8 degrees Celsius
- Do not use hemolyzed or contaminated samples
- Perform quality controls, positive and negative, included in the kit. Follow procedures on test kit
Testing procedures for mono?
- Allow the Latex Reagent and controls to reach room temperature (20 to 30 degrees C)
- Gently shake the Latex Reagent vial to disperse and suspend the latex particles in the buffer solution. Vigorous shaking should be avoided
- Use the dispenstir to transfer the specimen to the card, dispense 1 drop
- Add one drop of Latex Reagent next to the drop of sample
- Mix both drops with stirrer covering the whole surface of the slide section
- Gently rotate the slide for 3 minutes manually or on a rotatory shaker set at 60-100 rpm
- Look for the presence or absence of agglutination after the aforementioned period of time
- Report results as either positive or negative
- Monospot Test Results
solution
Positive result/Negative result?
- Shows agglutination
* Demonstrates a smooth, homgoenous
How is the sample stored in refrigeration?
2-8 degrees celcius
When must the sample be tested?
Within 24 hours if stored at 2-8 C
How is the latex reagen vial shaken?
GENTLY to disperse and suspend latex particles in buffer solution
How is the slide rotated?
3 minutes mannualy or rotator shaker at 60-100 rpm
What are you looking for on the monospot test?
presence or absence of AGGLUTINATION after 3 minutes
How are results reported?
Positive or Negative
What is a positive result?
Agglutination
What controls are used for monospot?
Positive and negative controls
