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Lab Part 2 > RPR > Flashcards

RPR Flashcards

1
Q

What is a non-treponemal test for serologic detection of the antibody reagin, which is produced in response to an infection (syphilis) caused by Treponema pallidum?

A

Rapid Plasma Reagin

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2
Q

What is produced in response to an infection (syphilis) caused by Treponema pallidum?

A

Antibody Reagin (phospholipid)

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3
Q

So what is RPR testing for?

A

Syphilis

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4
Q

Where is RPR found?

A

Serum or plasma of patients with syphilis

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5
Q

What is used as a vehicle/antigen to see results macroscopically?

A

Carbon Charcoal

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6
Q

Does RPR required confirmation?

A

Yes (therefore it is a screening test)

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7
Q

Fluorescent treponemal antibody-absorbed test (FTA-ABS)

A

Dead T. pallidum is fixed on a slide, and patient’s serum is added

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8
Q

FTA ABS (FAT ABS) is what?

A

Confirmatory test for RPR

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9
Q

FAT ABS detects antibody how?

A
  • By addition of fluorescein-labeled anti-human globulin.

* under ultraviolet microscope, it will fluoresce if positive

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10
Q

What bacteria is associated with RPR?

A

Treponema pallidum

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11
Q

What lipid is the antibody regain?

A

Phospholipid

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12
Q

Non-reactive test results on rpr show?

A

No flocculation

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13
Q

What is the monospot test used for?

A

detect infectious mononucleosis which is a self limiting disease caused by the Epstein-Barr virus

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14
Q

When is mono typically seen?

A

young adults and children less than 5 years of age

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15
Q

How does mono present?

A

fever, malaise, lethargy, sore throat with exudates, enlarged lymph nodes on the neck, mild hepatitis, enlarged spleen, and occasional blotchy skin rash

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16
Q

What accumulation does mono cause?

A

increased numbers and abnormal forms of lymphocytes and monocytes in the lymph nodes

17
Q

What antibody is formed due to mono?

A

Heterophil

18
Q

What is a heterophil antibody?

A

Copy of the present antibody

19
Q

How is serum sample taken for rpr?

A
  • Collect venous blood in tubes without anticoagulant (red top and sst)
  • After centrifugation, serum should be clear and unhemolyzed
20
Q

What test is performed for RPR?

A

RPR card test kit

21
Q

Prior to using test card kit what must you do for rpr?

A
  • Centrifuge the specimen (serum or plasma) to sediment cellular elements
  • Mix the antigen suspension, prior to opening ampule, by shaking vigorously for 10-15 seconds
  • Attach the needle to the hub of the dispensing bottle. Ensure the antigen is below the break line
  • Snap off the top of the ampule and draw all of the antigen into the dispensing bottle
22
Q

How do you mix the antigen suspension in the card kit for rpr?

A
  • Shake VIGOROUSLY for 10-15 seconds
23
Q

What must be used as a confirmatory test for RPR card kit test?

A

FAT ABS (FTA-ABS)

24
Q

What is the speed and time the card kit spins on the rotator for rpr?

A

8 minutes at 100 revolutions per minute

25
Q

How often do you rotate the card to differentiate the non-reactive from the weakly reactive results?

A

3 or 4 to and from

26
Q

What are the results on the test kit for rpr?

A

Reactive or Non-reactive

27
Q

How do you know it’s reactive?

A

Flocculation is present

28
Q

When are Rapid Plasma Reagin controls conducted?

A

always be run with each batch of Rapid Plasma Reagin tests

29
Q

How does quality control start?

A

with proper collection and handling of specimen

30
Q

How is quality control divided?

A
  • Equipment quality control

* Reagent quality control

31
Q

What is the mechanical rotor calibrated to?

A

100 rpm

32
Q

What is the antigen needle calibrated to?

A

60 drops per 1 ml

33
Q

What controls are used for rpr?

A
  • reactive
  • weak-reactive
  • non-reactive
34
Q

How are controls treated?

A

As patients and run every time an unknwon sample is tested

Lab Part 2 (13 decks)

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