MOUNTING

Question 1

purpose of mounting

Answer 1

plus coverslipping
preserve slide
protect tissue from trauma

Question 2

what does mounting and coverslipping provide

Answer 2

maxium degree of transparency to stained tissue sections

Question 3

what affects the details of tissue

Answer 3

light refraxtion of the slide and the tissue
tissue components

Question 4

when tissue photography will be considered, what matters most?

Answer 4

refractive index of the mountant

Question 5

lower objective:____ apreture

Answer 5

wider

Question 6

mounting increases the refractive index by

Answer 6

1.518 RI

Question 7

RI of glass slide and coverslip, immersion oil

Answer 7

1.51

Question 8

RI of section

Answer 8

1.38-1.46

Question 9

characteristics of mountants used nowadays

Answer 9

commercial, non-aqueous

Question 10

what can be used as mountant in a rush

Answer 10

distillled water

Question 11

what do you expect when youll use distilled water?

Answer 11

aberrations

Question 12

most water based mounting media have __ in their recipe due to its RI of 1.47

Answer 12

glycerol

Question 13

mounting media ideal for resin-embedded slides

Answer 13

resinous

Question 14

resinous media also ideal for slide sections cleared in

Answer 14

xylene , toluene, benzene

Question 15

RI 1.33
stains may bleed, not be stored extensively

Answer 15

distilled water

Question 16

RI 1.46
fat stains

Answer 16

glycerin

Question 17

RI 1.47
best view

Answer 17

glycerin jelly

Question 18

RI 1.52
fluorescent microscopy

Answer 18

apathys medium

Question 19

RI 1.43
gum arabic component
doesnt solidify upon storage

Answer 19

farrants medium

Question 20

rec for mounting frozen sections

Answer 20

bruns fluid

Question 21

resinous is ____ than aqueous

Answer 21

superior

Question 22

RI 1.524
from Abus balsamee tree
best

Answer 22

canada balsam

Question 23

alternative for DPX

Answer 23

clarite

Question 24

to keep slides permanently, some mountants may require

Answer 24

ringing

Question 25

ringing means

Answer 25

coating margin with liquid prep to seal sides

Question 26

ringing prevents drying out, imortant for

Answer 26

aqueous mounting media

Question 27

paraffin w. ringing rion

Answer 27

temporary

Question 28

nail polish

Answer 28

months

Question 29

kronig cement

Answer 29

2 parts paraffin 4-9 parts powdered colophonium resin

Question 30

cellulose adhesives

Answer 30

durofix

Question 31

repair: slide covered with what 6:1 ratio of

Answer 31

butyl acetate and durofix