MPS Technology Part I

Question 1

What is a genome

Answer 1

The entirety of an organisms hereditary information (can be DNA or RNA)

Question 2

Whag is a core genome

Answer 2

The genes that are present in all seqeunced strains of a species

Question 3

What is an accessory genome

Answer 3

All of the genetic elements that are not part of the core genome, including plasmids

Question 4

What is a pangenome

Answer 4

The set of genes encoded by the genomes of all strains Kd a species (including the core genome and accessory genomes of all strains of a species)

Question 5

What is a draft genome

Answer 5

A genomic dna seqeunce of relatively low accuracy that has some missing segments (gaps) or contigs that are in the wrong order or orientation

Question 6

What is a complete genome

Answer 6

A genomic dna sequence of relatively high accuracy that has no ambiguity about the order or orientation of any segment and has a few if any gaps

Question 7

How does whole genome shotgun sequencing work

Answer 7

It’s a better way than doing cloning sequencing where you used to have the fragmented dna in a vector and and seqeuncing it with the vector

Shotgun:

DNA is randomly fragmented

The fragmented pieces are seqeunced to find order of bases

The computer programs assemble those fragmented seqeunces and close the gaps (hard to close the gaps)

Then edit the final seqeunce and annotate it