Mutagenesis Flashcards
What light conditions cause Arabidopsis to be etiolated / de-etiolated?
Etiolation:
- Darkness
De-etiolation:
- White light
- Far-red (HIR)
- Red (LFR)
- Blue (cryptochrome)
What happens with loss of PhyA?
Alters effect of far-red and blue light on seedlings
What happens with loss of PhyB?
Alters effect of red and blue light on seedlings
What happens with loss of PhyA and PhyB (PhyAB mutant)?
Alters effect of far-red, red and blue light on seedlings
What can be concluded from looking at the effects of PhyA mutants and PhyB mutants?
PhyA controls far-red HIR and interacts with blue light receptors
PhyB controls red / far-red LFR
How can reverse genetics allow removal of all phytochromes?
TDNA is randomly inserted into genes
Plants with inactive genes are identified and all mutants are crossed together.
What does the blu mutant do?
blu mutant de-etiolates under white light but not blue light.
What is PhyB blind to?
Red light
What characteristics to PhyB mutants have?
Long petioles and flower early.
What is a cryptochrome?
Blue light receptor
What do cop/det mutants do?
De-etiolate in darkness
What happens to etioplasts and stomata of dark grown, etiolated plants?
Etioplast: not photosynthetically competant
Stomata: not properly developed, photosynthetic genes not expressed.
What are brassinosteroids?
Steroid hormone required for development and growth of plants.
What happens when brassinosteroids are applied to det mutants?
Det mutants behave like wildtype plants.
