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Flashcards in mycology lab quiz Deck (27)
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1
Q

tinea unguium

A

infection of the nail

2
Q

tinae corporis

A

body

3
Q

tinae pedis

A

foot

4
Q

tinae barbay

A

beard

5
Q

tinae cruris

A

groin

6
Q

what does the word tinae mean?

A

latin word for ringworm

7
Q

name the short lateral structure that will develop when certain yeast are incubated at 37 degrees in a rich medium

A

germ tube

8
Q

name one yeast that is germ tube positive

A

candida albicans

9
Q

at what temperature should chlamydospore agar be incubated?

A

25 degrees

room temp.

10
Q

where should the technologist look for spores on a chlamydospore agar plate?

A

edge of the coverslip

11
Q

what color and shape are the spores on a chlamydospore plate?

A

blue and round

12
Q

what is the principle of the india ink preparation

A

drop of india ink and drop of organism
then look on high power will see halo around the budding yeast because particles of the india ink bounce off the unstained capsule

13
Q

what organism is india ink especially used for?

A

cryptococcus neoformans

14
Q

what type of specimen is most frequently used for india ink ?

A

CSF

15
Q

what reagent should be used for the examination of the reproductive structures of molds that are already growing in a culture?

A

lactophenol cotton blue (LPCB)

16
Q

what technique can be used to “save” wet preps for future use?

A

clear fingernail polish all around coverslip

17
Q

name several media used for the primary isolation of fungi

A

sabsC
SDA
sabourauds
BHI

18
Q

why would you incubate a culture for fungi at 25 degrees and 37 degrees

A

to demonstrate both phases (dimorphic/diphasic)

mold is at 25 and yeast is at 37

19
Q

what term is used for fungi that grow differently at these temperatures

A

dimorphic

20
Q

why do mycologists use KOH

A

to clear keratin so fugal elements will stand out

21
Q

what specific protein does KOH affect?

A

keratin

22
Q

name at least two fungal elements that could be seen in a KOH prep

A
hyphae
pseudohyphae
arthrospores
blastospores
budding yeast
23
Q

what is the difference between ectothrix and endothrix hair invasion

A

ectothrix is outside the hair shaft

endothrix is inside the hair shaft

24
Q

name one genus of fungus which causes ringworm

A

microsproum
epidermophyton
trichophyton

25
Q

name one genus of fungus which exhibits dimorphism

A

blastomyces
coccidiodes
paracoccidiodes
histoplasma sporothrix

26
Q

name of genus of fungus which is a frequent contaminant

A

aspergillus

penicillium

27
Q

what is a slide culture?

A

a fungus on slab of agar with cover slip allowing to grow on cover slip then consequently viewing perfect morphology
best way to observe fungi in natural state