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Flashcards in mycology lab quiz Deck (27)
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1

tinea unguium

infection of the nail

2

tinae corporis

body

3

tinae pedis

foot

4

tinae barbay

beard

5

tinae cruris

groin

6

what does the word tinae mean?

latin word for ringworm

7

name the short lateral structure that will develop when certain yeast are incubated at 37 degrees in a rich medium

germ tube

8

name one yeast that is germ tube positive

candida albicans

9

at what temperature should chlamydospore agar be incubated?

25 degrees
room temp.

10

where should the technologist look for spores on a chlamydospore agar plate?

edge of the coverslip

11

what color and shape are the spores on a chlamydospore plate?

blue and round

12

what is the principle of the india ink preparation

drop of india ink and drop of organism
then look on high power will see halo around the budding yeast because particles of the india ink bounce off the unstained capsule

13

what organism is india ink especially used for?

cryptococcus neoformans

14

what type of specimen is most frequently used for india ink ?

CSF

15

what reagent should be used for the examination of the reproductive structures of molds that are already growing in a culture?

lactophenol cotton blue (LPCB)

16

what technique can be used to "save" wet preps for future use?

clear fingernail polish all around coverslip

17

name several media used for the primary isolation of fungi

sabsC
SDA
sabourauds
BHI

18

why would you incubate a culture for fungi at 25 degrees and 37 degrees

to demonstrate both phases (dimorphic/diphasic)
mold is at 25 and yeast is at 37

19

what term is used for fungi that grow differently at these temperatures

dimorphic

20

why do mycologists use KOH

to clear keratin so fugal elements will stand out

21

what specific protein does KOH affect?

keratin

22

name at least two fungal elements that could be seen in a KOH prep

hyphae
pseudohyphae
arthrospores
blastospores
budding yeast

23

what is the difference between ectothrix and endothrix hair invasion

ectothrix is outside the hair shaft
endothrix is inside the hair shaft

24

name one genus of fungus which causes ringworm

microsproum
epidermophyton
trichophyton

25

name one genus of fungus which exhibits dimorphism

blastomyces
coccidiodes
paracoccidiodes
histoplasma sporothrix

26

name of genus of fungus which is a frequent contaminant

aspergillus
penicillium

27

what is a slide culture?

a fungus on slab of agar with cover slip allowing to grow on cover slip then consequently viewing perfect morphology
best way to observe fungi in natural state