NEUR 2035 Final - Lab 6

Question 1

True or false:

When we measure PKA activity, we are actually measuring the amount of PKA protein in the sample.

Answer 1

False

Question 2

When we use ELISA to measure PKA activity, we will use a primary antibody that binds to…
A. Active PKA
B. Phosphorylated serine and/or threonine
C. PKA

Answer 2

B

Question 3

When we do a 2-fold dilution of Sample A, if we add 100 μL of sample A to the test tube, then we need to add ____ μL of water.
A. 200
B. 400
C. 100
D. 300

Answer 3

C

Question 4

When we do a 3-fold dilution of Sample A, if we add 100 μL of sample A to the test tube, then we need to add ____ μL of water.
A. 200
B. 400
C. 300
D. 100

Answer 4

A

Question 5

True or false:

When we measure PKA activity, we are actually measuring the amount of substrate (amino acid) that gets phosphorylated by PKA in the sample.

Answer 5

True

Question 6

During ELISA, the substrate (amino acid) will be…
A. Coated in each well of a 96-well plate
B. Added to each well of a 96-well plate

Answer 6

A

Question 7

PKA can add a phosphate group to the amino acids….

Answer 7

Serine and/or threonine

Question 8

How do we measure the amount of primary antibody that has bound to the phosphorylated amino acids during ELISA?

Answer 8

A secondary antibody is bound to the primary antibody. The secondary antibody contains an enzyme that reacts with a color-changing substrate. We then measure the color intensity with a spectrophotometer or plate reader.

Question 9

What does ELISA stand for?

Answer 9

Enzyme-Linked Immunosorbent Assay