new previously unknown-LEARN Flashcards
LC3 is related to
autophagy
LC3
a protein that affects the myosin light chain
type 3 secretory systems e.g. shigella
membrane remnants from when shigella has escaped other cells triggers autophagy. The shigella releases IscB via the type III secretory system and this represses autophagy
anti-microbial methods of phages (3)
1) CRISPR
2) LexA3
3) lytic pathway
LexA3
the phage injects this into the bacteria. LexA3 stops the SOS response (used to fix DNA after fluorquinolones have been added). This makes antibiotics more effective at killing cells
LexA3 main ideas
use phage to deliver a protein which interferes with essential bacterial processes rendering it more susceptible to antibiotics
faecal extracts inhibit the expression of
salmonella pathogenicity island-1
SPI-1 (salmonella pathogenicity island-1)
inhibits specific ATPase of type 3 secretory systems of salmonella, which cause infection
synergsistc
when more than one antibiotic is used to treat an infection
why are synergistic antibiotic not that great
increase the number of resistance bacteria- less competition
another anti-microbial strategy e.g. UPEC
mannose added, which has a higher affinity to bacterial pilli than the mannose on the host cell wall. prevents them from attaching to the cell and causing disease
virstatin
a small molecule which inhibits the transcription of ToxT in Vibrio Cholerae
ToxT is a
Toxin of V.cholerae
anti-virulence strategies
inhibit specific mechanisms that promote infection and are essential for persistence
which Proteins are responsible for host lysis and vision burst into the environment
lysins, holding and murrain synthesis inhibitors
IscB
a protein secreted by the Type III secretory system which represses autophagy–> prevents autophagsome formation
what prevents autophagosome formation in shigella
injection of IscB via Type III secretory system
pH of early endosome
6.5
pH of phagolsosome
5.5
protein in early endosome
Rab5 and EEA1
protein in phagolysosome
cathepsin and Lamps
how can macrophages recognise bacteria in vesicles
TLRs e.g. Nod-like receptors
TLR found in SCV
TLR 9 - protects and allows for growth of salmonela
shigella flexneri cause host cell death by
secretions to T3SS efectors- recognised by NLRs and cause pyroptosis- form of necrotic cell death
