Non-fermenters

Question 1

media we can use to group non fermenters

Answer 1

OF media and TSI

Question 2

non fermenters are gram ______

Answer 2

gram negative

Question 3

shape of non fermenters

Answer 3

bacilli or coccobacilli

Question 4

The non-fermenters are grouped together based on
their inability to _____carbohydrates, that is to produce
acid from sugars anaerobically

Answer 4

ferment

Question 5

Some non-fermenters can utilize sugars aerobically
and they are termed __

Answer 5

oxidizers

Question 6

Others do not utilize or
break down carbohydrates at all and they are called

Answer 6

asaccharolytic or non-oxidizers.

Question 7

test that can differentiate non fermenters from Enterobacteriaceae

Answer 7

oxidase test

Question 8

result of non fermenters in oxidase test

Answer 8

positive

Question 9

clinical infections of nonfermenters

Answer 9

septicemia, meningitis, osteomyelitis, and
wound infection

Question 10

The non-fermenters are notoriously known for
colonizing or infecting hospitalized patients and the immunocompromised. That’s why we can classify them as (opportunistic or true pathogen) ?

Answer 10

opportunistic

Question 11

Species under this group also tend to be ___ to
multiple classes of antibiotics

Answer 11

resistant

Question 12

result of non fermenters in slant or butt of TSI or LIA

Answer 12

K/K No acid production

Question 13

GIVE THE CHARACTERISTIC OF Pseudomonads
gram _____
type of respiration:
motility:
oxidase:
catalase:
mcconkey:
oxidizer or nonoxidizer:
spore forming capability:

Answer 13

gram negative bacillus
aerobic
motile
oxidase +
catalase +
grows on mcconkey
oxidizer
non-spore forming

Question 14

genus pseudomonas are catalase positive except

Answer 14

Pseudomonas luteolus and P. oryzihabitan

Question 15

● Most commonly isolated species under genus pseudomonas

Answer 15

pseudomonas aeruginosa

Question 16

the leading cause of
nosocomial respiratory tract infections

Answer 16

Pseudomonas aeruginosa

Question 17

is a disease characterized by thick
and sticky mucus which can clog the lungs. The
secretions

Answer 17

Cystic fibrosis

Question 18

Pseudomonas aeruginosa is also referred to as the agent of ___ because wound and burn infections typically give rise to blue-green colored pus.

Answer 18

blue pus

Question 19

Otitis caused by P. aeruginosa is sometimes referred to as ___. That is why it has been implicated in ear infections, especially in
individuals with aquatic exposure such as swimmers and
divers

Answer 19

swimmer’s ear.

Question 20

P. aeruginosa also a known cause of_____, a type of dermatitis commonly found in users
of such recreational facilities.

Answer 20

jacuzzi or hot tub
syndrome

Question 21

P. aeruginosa also causes ____associated with the use of contaminated contact lens solution.

Answer 21

keratitis

Question 22

Virulence factors of P. aeruginosa

Answer 22

Endotoxin (lipopolysaccharide), motility, pili, capsule, and exotoxins

Question 23

most important exotoxin for P. aeruginosa is

Answer 23

exotoxin A

Question 24

How exotoxin A works?

Answer 24

functions similarly to diphtheria toxin by blocking protein synthesis

Question 25

Members of the Pseudomonas fluorescence group produce ___, which is a yellow green or yellow brown pigment.

Answer 25

pyoverdine

Question 26

Most strains of P. aeruginosa will also produce a blue pigment called ___

Answer 26

pyocyanin

Question 27

a pigment that is unique to P. aeruginosa

Answer 27

pyocyanin

Question 28

what type of hemolysis does P.aeruginosa produced in BAP?

Answer 28

b-hemolysis

Question 29

odor produced by P.aeruginosa

Answer 29

produce a fruity, grape-like odor

Question 30

the odor produced by P. aeruginosa is caused by the presence of

Answer 30

2-aminoacetophenone.

Question 31

SCA test result of P. aeruginosa

Answer 31

citrate positive

Question 32

is a medium used in identification of P. aeruginosa

Answer 32

Cetrimide agar

Question 33

P. aeruginosa ; When isolated from a cystic fibrosis patient, ___colonies are sometimes observed.

Answer 33

mucoid

Question 34

P. aeruginosa is resistant to :

Answer 34

Resistant: - Penicillin - Ampicillin - 1st and 2nd generation cephalosporins - Trimethoprim-sulfamethoxazole - Chloramphenicol - Tetracyclines

Question 35

P. aeruginosa is susceptible to

Answer 35

Susceptible: - Aminoglycosides - Semisynthetic penicillins - 3rd and 4th generation cephalosporins - Carbapenems and fluoroquinolones

Question 36

Isolated from respiratory specimen, contaminated blood products, urine, cosmetics, hospital equipment, and fluids

Answer 36

P. fluorescens and P. putida

Question 37

P. fluorescens and P. putida can grow at what temperature

Answer 37

4 degree celcius

Question 38

they have been linked to transfusion-associated septicemia.

Answer 38

Pseudomonas fluorescens and Pseudomonas putida

Question 39

P. fluorescens and P. putida Produce acid from ___

Answer 39

xylose

Question 40

P. fluorescens and P. putida are differentiated by ___

Answer 40

gelatin hydrolysis

Question 41

Gelatin hydrolysis can be used to differentiate the two species from each other; _____ is negative and _____ is positive

Answer 41

P. putida is negative and P. fluorescens is positive

Question 42

P. fluorescens and P. putida are resistant to

Answer 42

carbenicillin and SXT

Question 43

P. fluorescens and P. putida are susceptible to

Answer 43

aminoglycosides, polymyxin, and piperacillin

Question 44

Characterized by wrinkled, leathery, adherent colonies that may produce a light-yellow or brown pigment

Answer 44

P. stutzeri

Question 45

type of hemolysis of acinetobacter

Answer 45

not applicable, both species of acinetobacter is non-hemolytic

Question 46

2 species of acinetobacter

Answer 46

A. baumanii and A. lwoffii

Question 47

Acinetobacter ____is capable of oxidizing glucose while Acinetobacter ____ cannot.

Answer 47

Acinetobacter baumanii is capable of oxidizing glucose while Acinetobacter lwoffii cannot.

Question 48

characteristics of acinetobacter oxidase: catalase: motility: hemolysis: respiration:

Answer 48

oxidase negative catalase positive nonmotile non-hemolytic strictly aerobic

Question 49

A. ____is saccharolytic, A. ____ is assacharolytic

Answer 49

A. baumanii is saccharolytic, A. lwoffii is assacharolytic

Question 50

A. _____is resistant to penicillins, 1st and 2nd generation cephalosporins, and fluoroquinolones

Answer 50

baumanni

Question 51

A. ____ is susceptible to almost all antimicrobials

Answer 51

lwofii

Question 52

This bacteria can grow in routine isolation media such as MacConkey agar, in which they sometimes appear as purplish colonies (which resembles a lactose fermenting organism)

Answer 52

acinetobacter

Question 53

is the third most common nonfermentative, gram-negative bacillus isolated in the clinical laboratory.

Answer 53

Stenotrophomonas maltophilia

Question 54

● When isolated, it is initially regarded as a saprophyte or colonizer, instead of a pathogen

Answer 54

Stenotrophomonas maltophilia

Question 55

Appears bluish on MacConkey

Answer 55

S. maltophilia

Question 56

S. maltophilia are susceptible to

Answer 56

SXT

Question 57

Its name collectively means affinity to malt which refers to the acid production of S. maltophilia from ____and not glucose

Answer 57

maltose

Question 58

The ____ contains plant pathogens that have arisen as opportunistic organisms

Answer 58

Burkholderia cepacia complex

Question 59

Burkholderia were Usually associated with patients who has ___

Answer 59

pneumonia with cystic fibrosis or chronic granulomatous disease

Question 60

detects the production of the enzyme β-galactosidase. The positive result is indicated by the development of a yellow color after incubation

Answer 60

o-nitrophenyl-β-Dgalactopyranoside or ONPG (+)

Question 61

Burkholderia are resistant to aminoglycosides and polymyxins

Answer 61

and are susceptible to chloramphenicol, ceftazidime, piperacillin, minocycline, fluoroquinolones, and SXT

Question 62

can burkholderia grow on McConkey agar?

Answer 62

yes

Question 63

describe the colonies of burkholderia

Answer 63

colonies are non-wrinkled, and can produce a non fluorescing yellow or green pigment

Question 64

causes glander's disease, a respiratory tract zoonosis primarily affecting livestocks such as horses, mules, and donkeys.

Answer 64

B. mallei

Question 65

This organism is considered to be a potential bioterrorism agent.

Answer 65

B. mallei

Question 66

B. mallei is resistant to

Answer 66

polymyxin

Question 67

B. mallei Treatment includes ____

Answer 67

ceftazidime, imipenem, doxycycline, and tetracycline

Question 68

causes melioidosis, a pulmonary disease; a glandulars-like disease

Answer 68

B. pseudomallei

Question 69

also called as vietnamese time bomb

Answer 69

B. pseudomallei

Question 70

describe the colony of pseudomallei

Answer 70

The colonies appear wrinkled, much like Pseudomonas stutzeri. P. stutzeri, however, does not utilize lactose in contrast with B. pseudomallei

Question 71

The organism would typically demonstrate bipolar staining with gram stain which can be described as having a safety pin appearance

Answer 71

B. pseudomallei

Question 72

medium used to identify B. pseudomallei

Answer 72

Ashdown medium, resulted to a deep pink colony with earthy odor

Question 73

B. pseudomallei includes Treatment

Answer 73

includes SXT and fluoroquinolones