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BACTE - FINALS > Non-fermenters > Flashcards

Non-fermenters Flashcards

1
Q

media we can use to group non fermenters

A

OF media and TSI

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2
Q

non fermenters are gram ______

A

gram negative

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3
Q

shape of non fermenters

A

bacilli or coccobacilli

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4
Q

The non-fermenters are grouped together based on
their inability to _____carbohydrates, that is to produce
acid from sugars anaerobically

A

ferment

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5
Q

Some non-fermenters can utilize sugars aerobically
and they are termed __

A

oxidizers

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6
Q

Others do not utilize or
break down carbohydrates at all and they are called

A

asaccharolytic or non-oxidizers.

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7
Q

test that can differentiate non fermenters from Enterobacteriaceae

A

oxidase test

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8
Q

result of non fermenters in oxidase test

A

positive

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9
Q

clinical infections of nonfermenters

A

septicemia, meningitis, osteomyelitis, and
wound infection

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10
Q

The non-fermenters are notoriously known for
colonizing or infecting hospitalized patients and the immunocompromised. That’s why we can classify them as (opportunistic or true pathogen) ?

A

opportunistic

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11
Q

Species under this group also tend to be ___ to
multiple classes of antibiotics

A

resistant

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12
Q

result of non fermenters in slant or butt of TSI or LIA

A

K/K No acid production

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13
Q

GIVE THE CHARACTERISTIC OF Pseudomonads
gram _____
type of respiration:
motility:
oxidase:
catalase:
mcconkey:
oxidizer or nonoxidizer:
spore forming capability:

A

gram negative bacillus
aerobic
motile
oxidase +
catalase +
grows on mcconkey
oxidizer
non-spore forming

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14
Q

genus pseudomonas are catalase positive except

A

Pseudomonas luteolus and P. oryzihabitan

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15
Q

● Most commonly isolated species under genus pseudomonas

A

pseudomonas aeruginosa

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16
Q

the leading cause of
nosocomial respiratory tract infections

A

Pseudomonas aeruginosa

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17
Q

is a disease characterized by thick
and sticky mucus which can clog the lungs. The
secretions

A

Cystic fibrosis

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18
Q

Pseudomonas aeruginosa is also referred to as the agent of ___ because wound and burn infections typically give rise to blue-green colored pus.

A

blue pus

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19
Q

Otitis caused by P. aeruginosa is sometimes referred to as ___. That is why it has been implicated in ear infections, especially in
individuals with aquatic exposure such as swimmers and
divers

A

swimmer’s ear.

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20
Q

P. aeruginosa also a known cause of_____, a type of dermatitis commonly found in users
of such recreational facilities.

A

jacuzzi or hot tub
syndrome

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21
Q

P. aeruginosa also causes ____associated with the use of contaminated contact lens solution.

A

keratitis

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22
Q

Virulence factors of P. aeruginosa

A

Endotoxin (lipopolysaccharide), motility, pili, capsule, and exotoxins

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23
Q

most important exotoxin for P. aeruginosa is

A

exotoxin A

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24
Q

How exotoxin A works?

A

functions similarly to diphtheria toxin by blocking protein synthesis

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25
Q

Members of the Pseudomonas fluorescence group produce ___, which is a yellow green or yellow brown pigment.

A

pyoverdine

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26
Q

Most strains of P. aeruginosa will also produce
a blue pigment called ___

A

pyocyanin

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27
Q

a pigment that is unique to P. aeruginosa

A

pyocyanin

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28
Q

what type of hemolysis does P.aeruginosa produced in BAP?

A

b-hemolysis

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29
Q

odor produced by P.aeruginosa

A

produce a fruity, grape-like
odor

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30
Q

the odor produced by P. aeruginosa is caused by the presence of

A

2-aminoacetophenone.

31
Q

SCA test result of P. aeruginosa

A

citrate positive

32
Q

is a medium used in identification of P. aeruginosa

A

Cetrimide agar

33
Q

P. aeruginosa ;
When isolated from a cystic fibrosis patient, ___colonies are sometimes observed.

A

mucoid

34
Q

P. aeruginosa is resistant to :

A

Resistant:
- Penicillin
- Ampicillin
- 1st and 2nd generation cephalosporins
- Trimethoprim-sulfamethoxazole
- Chloramphenicol
- Tetracyclines

35
Q

P. aeruginosa is susceptible to

A

Susceptible:
- Aminoglycosides
- Semisynthetic penicillins
- 3rd and 4th generation cephalosporins
- Carbapenems and fluoroquinolones

36
Q

Isolated from respiratory specimen, contaminated
blood products, urine, cosmetics, hospital equipment,
and fluids

A

P. fluorescens and P. putida

37
Q

P. fluorescens and P. putida can grow at what temperature

A

4 degree celcius

38
Q

they have been linked to transfusion-associated
septicemia.

A

Pseudomonas fluorescens and Pseudomonas putida

39
Q

P. fluorescens and P. putida Produce acid from ___

A

xylose

40
Q

P. fluorescens and P. putida are differentiated by ___

A

gelatin hydrolysis

41
Q

Gelatin hydrolysis can be used to differentiate the two species from each other; _____ is negative and _____ is positive

A

P. putida is negative and P.
fluorescens is positive

42
Q

P. fluorescens and P. putida are resistant to

A

carbenicillin and SXT

43
Q

P. fluorescens and P. putida are susceptible to

A

aminoglycosides, polymyxin, and
piperacillin

44
Q

Characterized by wrinkled, leathery, adherent colonies
that may produce a light-yellow or brown pigment

A

P. stutzeri

45
Q

type of hemolysis of acinetobacter

A

not applicable, both species of acinetobacter is non-hemolytic

46
Q

2 species of acinetobacter

A

A. baumanii and A. lwoffii

47
Q

Acinetobacter ____is capable of oxidizing glucose while Acinetobacter ____ cannot.

A

Acinetobacter baumanii is capable of oxidizing glucose while Acinetobacter lwoffii cannot.

48
Q

characteristics of acinetobacter
oxidase:
catalase:
motility:
hemolysis:
respiration:

A

oxidase negative
catalase positive
nonmotile
non-hemolytic
strictly aerobic

49
Q

A. ____is saccharolytic, A. ____ is assacharolytic

A

A. baumanii is saccharolytic, A. lwoffii is assacharolytic

50
Q

A. _____is resistant to penicillins, 1st and 2nd generation cephalosporins, and fluoroquinolones

A

baumanni

51
Q

A. ____ is susceptible to almost all antimicrobials

A

lwofii

52
Q

This bacteria can grow in routine isolation media such as MacConkey agar, in which they sometimes appear as purplish colonies (which resembles a lactose fermenting
organism)

A

acinetobacter

53
Q

is the third most
common nonfermentative, gram-negative bacillus
isolated in the clinical laboratory.

A

Stenotrophomonas maltophilia

54
Q

● When isolated, it is initially regarded as a saprophyte
or colonizer, instead of a pathogen

A

Stenotrophomonas maltophilia

55
Q

Appears bluish on MacConkey

A

S. maltophilia

56
Q

S. maltophilia are susceptible to

A

SXT

57
Q

Its name
collectively means affinity to malt which refers to the acid production of S. maltophilia from ____and not glucose

A

maltose

58
Q

The ____ contains plant pathogens that have arisen as opportunistic organisms

A

Burkholderia cepacia complex

59
Q

Burkholderia were Usually associated with patients who has ___

A

pneumonia with
cystic fibrosis or chronic granulomatous disease

60
Q

detects the production of the enzyme
β-galactosidase. The positive result is indicated
by the development of a yellow color after
incubation

A

o-nitrophenyl-β-Dgalactopyranoside or ONPG (+)

61
Q

Burkholderia are resistant to aminoglycosides and
polymyxins

A

and are susceptible to chloramphenicol, ceftazidime,
piperacillin, minocycline, fluoroquinolones, and SXT

62
Q

can burkholderia grow on McConkey agar?

A

yes

63
Q

describe the colonies of burkholderia

A

colonies are non-wrinkled, and can produce a non fluorescing yellow or green pigment

64
Q

causes glander’s disease, a respiratory tract zoonosis primarily affecting livestocks such as horses, mules, and donkeys.

A

B. mallei

65
Q

This organism is considered to be a potential bioterrorism agent.

A

B. mallei

66
Q

B. mallei is resistant to

A

polymyxin

67
Q

B. mallei Treatment includes ____

A

ceftazidime, imipenem, doxycycline, and tetracycline

68
Q

causes melioidosis, a pulmonary disease; a glandulars-like disease

A

B. pseudomallei

69
Q

also called as vietnamese time bomb

A

B. pseudomallei

70
Q

describe the colony of pseudomallei

A

The colonies appear wrinkled, much like Pseudomonas stutzeri. P. stutzeri, however, does not utilize lactose in contrast with B. pseudomallei

71
Q

The organism would typically demonstrate bipolar staining with gram stain which can be described as having a safety pin appearance

A

B. pseudomallei

72
Q

medium used to identify B. pseudomallei

A

Ashdown medium, resulted to a deep pink colony with earthy odor

73
Q

B. pseudomallei includes Treatment

A

includes SXT and fluoroquinolones

BACTE - FINALS (5 decks)

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