Spore-Forming, Catalase-Positive Bacilli Flashcards

(84 cards)

1
Q

Bacillus is a gram _____ bacteria.

A

positive

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2
Q

All bacillus are motile, except for _______.

A

Bacillus anthracis

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3
Q

Bacillus is a (spore, non-spore former) bacteria.

A

spore former

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4
Q

Bacillus form spores aerobically and are catalase-_______.

A

catalase-positive

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5
Q

The causative agent of anthrax

A

Bacillus anthracis

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6
Q

These are the two virulence factors of Bacillus anthracis.

A

glutamic acid capsule and anthrax toxin

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7
Q

Three components of the toxin in Bacillus anthracis.

A

protective antigen, edema factor, lethal factor

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8
Q

Protective antigen + edema factor =

A

edema

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9
Q

Protective antigen + lethal factor =

A

death of host cells

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10
Q

The capsule protecting the organism from phagocytosis, is a polypeptide of ________.

A

D-glutamic acid

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11
Q

A common disease of live-stock.

A

A nthrax

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12
Q

These are the four forms of clinical infections of anthrax seen in humans.

A

cutaneous anthrax, inhalation anthrax, gastrointestinal anthrax, injectional anthrax.

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13
Q

Disease associated with inhalation anthrax.

A

Woolsorter’s disease and ragpicker’s disease

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14
Q

This results from the injection of spores into the soft tissues during administration of drugs of abuse.

A

injectional anthrax

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15
Q

A clinical infection that occurs when wounds are contaminated with spores acquired through skin cuts, abrasions, or insect bites.

A

cutaneous anthrax

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16
Q

Refers to the ingestion of Bacillus anthracis spores.

A

gastrointestinal anthrax

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17
Q

The mild form of Wool-sorter’s disease may last from ______ days.

A

2-3 days

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18
Q

This form of anthrax may start as a small pimple or papule at the site of infection, 2-3 days after exposure.

A

cutaneous anthrax

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19
Q

Lesions associated with cutaneous anthrax are called _______.

A

malignant pustule

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20
Q

This form of anthrax can be associated with necrotizing fasciitis, organ failure, shock, coma, and meningitis.

A

injectional anthrax

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21
Q

Three specimens which can be collected to help in the diagnosis of B. anthracis

A

malignant pustule, sputum, blood

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22
Q

B. anthracis is typically isolated from normally sterile sites such as _____________.

A

blood, lung tissue, CSF

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23
Q

Using a spore stain, the vegetative cells of B. anthracis stain ______, and the spores stain _______.

A

red, green

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24
Q

Incubation period of B. anthracis (hours and temp)

A

3-6 hours, 37 degrees Celsius

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25
Appearance of B. anthracis when examined microscopically. This is characterized as large spherical bacilli in chains.
string of pearls
26
Culture media used to isolate B. anthracis.
BAP
27
Is B. anthracis (hemolytic, non-hemolytic) on BAP?
non-hemolytic
28
Consistency of B. anthracis colonies.
tenacious consistency
29
What carbohydrate is being fermented by B. anthracis?
glucose
30
B. anthracis grows in (high, low) salt concentration of ____% NaCl.
high, 7%
31
pH level of B. anthracis
<6
32
B. anthracis is susceptible to what drug?
penicillin
33
This bacillus is a common cause of food poisoning and gastrointestinal disease and opportunistic infection in susceptible hosts.
Bacillus cereus
34
These are the two forms of food poisoning in B. cereus.
diarrheal and emetic
35
This form of food poisoning is self-limiting.
emetic
36
B. cereus is also known as ________.
Fried Rice Bacillus
37
Eye infections associated with B. cereus.
endophthalmitis and keratitis with abscess formation
38
Diseases associated with B. cereus.
meningitis, septicemia, endocarditis, osteomyelitis
39
This form of food poisoning is caused by heat stable enterotoxin.
emetic form
40
This form of food poisoning is from the ingestion of meat or poultry, vegetables and pasta.
diarrheal form
41
Incubation period of diarrheal form.
8-16 hours
42
This form of food poisoning is caused by heat labile enterotoxin.
diarrheal form
43
This form of food poisoning is associated with the ingestion of fried rice.
emetic form
44
Specimen used in diagnosing B. cereus.
suspected food
45
Cells per gram of food in B. cereus which confirms that food poisoning is caused by this organism.
>10^5
46
Media used to isolate B. cereus.
BAP
47
Hemolysis of B. cereus
Beta-hemolytic
48
Bacteria most commonly encountered as a laboratory contaminant.
Bacillus subtilis
49
Bacillus subtilis is also known as _______.
Hay Bacillus
50
Media used to isolate Bacillus subtilis.
BAP
51
Hemolytic pattern of B. subtilis
beta-hemolytic
52
anaerobic spore-forming bacilli known as clostridia.
clostridium
53
All Clostridium are non-encapsulate, except ______.
C. perfringens
54
Clostridium: catalase ________; gram ________ bacilli
negative, positive
55
The bacteria or the toxins of Clostridium usually enters the body though ingestion or open wounds that have been contaminated with _______.
soil
56
Diseases associated with Clostridium.
tetanus, gas gangrene (myonecrosis), botulism, food poisoning
57
Clostridium that is most commonly isolated in blood cultures.
C. perfringens
58
Two virulence factors of C. perfringens
alpha-toxin and enterotoxin
59
Virulence factor in C. perfringens that causes gastrointestinal illness.
enterotoxin
60
Virulence factor in C. perfringens that causes tissue necrosis.
alpha-toxin
61
C. perfringens hemolytic pattern: inner zone: ________; outer zone: _______
beta, alph
62
Two types of food poisoning in C. perfringens.
Type A and C
63
Causes myonecrosis or gas gangrene
C. perfringens
64
Type of food poisoning in C. perfringens that is more serious and can cause enteritis necroticans.
Type C
65
Type of food poisoning in C. perfringens which is mild and self-limiting GI illness.
Type A
66
Incubation period of Type A food poisoning.
8-30 hours
67
Incubation period of Type C food poisoning.
5-6 hours
68
Type of food poisoning in C. perfringens that is followed by necrotic inflammation of the small intestine that may lead to bowel perforation.
Type C
69
Fluid filled blisters in gas gangrene.
Bullae
70
Also known as canned food bacillus
C. botulinum
71
Virulence factor of Clostridium botulinum.
botulism toxin
72
Hemolytic pattern of C. botulinum
beta hemolytic
73
Clinical manifestations of C. botulinum may develop as early as _____ hours or as late as ____ days.
2 hours, 3-8 days
74
Possible treatments for C. botulinum
antitoxin, supportive care
75
Two types of botulism
Infant and Wound
76
Type of botulism that have clinical manifestations similar to that of the foodborne botulism.
Wound botulism
77
Contaminated honey is most implicated in this type of botulism.
Infant botulism
78
Also known as flappy baby syndrome.
Infant botulism
79
This is a soil and environmental inhabitant.
Clostridium tetani
80
Virulence factor of C. tetani.
tetanospasmin (neurotoxin) paralysis
81
C. tetani: gelatinase (positive, negative); lecithinase (positive, negative)
positive, negative
82
Cells with terminal spores in C. tetani appear as _____.
drumstick or tennis racket
83
This disease causes spastic type of paralysis with continuous muscle spasm leading to trismus or lockjaw.
tetanus
84
Vaccine used in treating tetanus.
diptheria-tetanus-acellular pertussis (DTaP) vaccine