Non-Protein Nitrogenous Substances Flashcards
In urea nitrogen determination, which method is inexpensive but lacks specificity? (Calbreath)
Colorimetric: diacetyl
In urea nitrogen determination, which method has greater specificity but is more expensive? (Calbreath)
Enzymatic: NH3 formation
Uric acid is ___% reabsorbed, and ___% excreted.
99; 1
Lesch-Nyhan syndrome is a deficiency of ____.
Hypoxanthine guanine phosphoribosyltransferase
Chemotherapy with ____ or ____ can decrease concentrations of uric acid.
Azathioprine; 6-mercaptopurine
In uric acid determination, which method produces problems with turbidity, and also has interference from several drugs? (Calbreath)
Colorimetric
In uric acid determination, which method needs special instrumentation and optical cells? (Calbreath)
Enzymatic: UV
In uric acid determination, which method has interference by reducing substances?
Enzymatic: H2O2 production
The BUN:creatinine ratio is ____.
10:1 to 20:1
In pre-renal disease, the BUN:creatinine ratio rises to ____.
> 20:1
What are the adsorbents and their composition, used for Jaffe reaction in creatinine determination?
Fuller’s earth - aluminum magnesium silicate
Lloyd’s - sodium aluminum silicate
In creatinine determination, which method is simple and non-specific? (Calbreath)
Colorimetric: end-point
In creatinine determination, which method is rapid and increases specificity? (Calbreath)
Colorimetric: kinetic
In creatinine determination, which method measures ammonia colorimetrically or with ISE? (Calbreath)
Enzymatic
Ammonia is removed from the circulation and converted to ____ in the liver.
Urea
