Nonenzymatic Protein Function and Protein Analysis Flashcards

Question

Ligand-Gated Channels

Answer 1

binding to a specific substance or ligand to cause channel to open or close neurotransmitters Km and vmax of enzymes apply here too

Answer 2

three primary protein domains membrane spanning - anchors receptor ligand binding - stimulated by ligand yo induce change catlyitc activateed by ligand binding domain changing leads to second messenger cascafe

Answer 3

family of integral membrane proteins involved in signal transduction characterized by 7 alpha helices receptors differ in specificity of the ligand binding area on extracellular surface

Answer 4

g proteins for GPCR with intracellular link GDP/GTP

Answer 5

Gs - stimulate adenylate cyclase to increase cAMP Gi - inhibit adenylate cyclase to decrease cAMP Gq - activates phospholipase C to leave phospholipid from PIP 2 to cleave from DAG and IP3 which then opens calcium channels

Answer 6

alpha binds to GDP and is complex with B and gamma when ligand binds to G protein, receptor is activasted and engages corresponding G protein GDp replaced with GTP and alpha dissociate from beta and gamma alpha alters adenylate cyclase activity alpha s activates alpha i enzyme is inhibited once GTP on alpha is dephosphorylated alpha will go back to B and hgamma and inactivate the protein

Answer 7

isolate proteins from smaller molecules before other isolation techniques

Answer 8

moves protein according to net charge and size velocity is migration velocity v = Ez/ f ``` E = e;ectroc field strength f = frictional coefficient z = net charge ```

Answer 9

used in electrophoresis small go fast, large go slow

Answer 10

mass to charge and mass to size ratios molecular size and charge of proteins

Answer 11

sodium dodecyyl sulfate proteins seperated on mass SDS disrupts non covalent interactions and negative charges only E affects velocity

Answer 12

seperation by isoelectric point pI pH when protein is neutal for individual amino acids this is zwitterion phase

Answer 13

fractioned through a porous matrix seperated by chemical and physical properties

Answer 14

sasmple on solid medium mobile phase for sample to elute out of

Answer 15

size and polarity have to role with how quickly compound goes less polar, the faster it goes pH, salinity, polarity

Answer 16

charged beads attract/bind opposin charge after all compounds are gone, salt is used to compete with charged protein to elute it out

Answer 17

beads have pores allowing small compounds to elute out slowly and large ones to elute out faster different MW are key here

Answer 18

coating stationary beads with item that has affinity for target like nickel for histags, antibodies, enzyme substrate, target is then washed off using elutant that competes for affinity of target letting it elute out eluent can also be certain pH or salinity to rid of ligand interaction on beads

Answer 19

protein is isolated and crystalized measures electron density and generates a pattern of dots to determine structure

Answer 20

protein structure

Answer 21

small protein analysis of AMINO ACIDS CLEAVAGE TO SEQ PROTEINS OF 50-70 protein hydrolysis removes N terminal amino acid one at a time

Answer 22

selectively cleaves proteins at specific amino acid residues to create smaller fragment for Edman/Gel electrophoresis positions cannot be determines as disulfide/salt bridges are broken

Answer 23

mixes protein in solution with coomassie blue dye dye gives protons to ionizable groups in protein to turn blue increase protein concentrations have larger concentration of blue dye standard curve created measuring absorbance and then unknown only one protein is effective

Nonenzymatic Protein Function and Protein Analysis Flashcards

(47 cards)