Nonhematopoietic Stem Cells

Question 1

Donor lymphocyte infusion

Purpose

Answer 1

Help to achieve long lasting remission after disease relapse after HSCT

Question 2

Donor lymphocyte infusion

Process

Answer 2

Lymphocytes collected by leukapheresis and aliquoted into doses

Question 3

Donor lymphocyte infusion

Dosage

Answer 3

Initial dose: typically 1x10^6 CD3+/kg recipient

Doses increase in CD3 count by half log or 1 log increments as much as 3 months apart

Question 4

Donor lymphocyte infusion

Most efficient for which disease

Answer 4

CML

Question 5

Donor lymphocyte infusion

Least effective with which disease

Answer 5

ALL

also only 15-40% of AML see response

Possibly due to more rapid proliferation of tumor cells

Question 6

Donor lymphocyte infusion

Complications

Answer 6

GVHD- possible increased opportunistic infections

Marrow aplasia

Question 7

Cytotoxic T cell infusion

Answer 7

Virus specific CTLs (CD8+) have successfully treated CMV and EBV (demonstrated to persist as long as 7 yrs in recipient)

Question 8

NK cell antigen markets

Answer 8

CD3-, CD56+

Question 9

Sipuleucel-T

Answer 9

Prostate cancer treatment from enriched autologous dendritic cells that have been activated ex vivo with recombinant protein

Question 10

MSC identification criteria

Answer 10

Adherence to tissue culture plastic

Positive: CD73, CD90, CD105
Negative:CD45, CD34, CD14, CD11b, CD79a, CD19, HLA-DR

Demonstrate in vitro differentiation to osteoblasts, adipocytes, and chondrocytes

Question 11

MSC HLA matching

Answer 11

Not necessary- do not induce GVHD or get rejected by recipient T cells