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Flashcards in NPN Deck (97)
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1

Which is true regarding NPN?
A. Compounds arise from the catabolism of proteins and nucleic acids
B. Majority are excreted in the kidneys
C. The term nonprotein nitrogen (NPN) originated in the early days of biochemistry
D. A and B

D

2

NPN of highest concentration in the blood and end product of protein catabolism state of hydration

Urea

3

First metabolite to increase in kidney diseases and part of the B:C Ratio (normal B:C ratio is 10:1-20:1)

Urea

4

increased BUN
a. poor nutrition
b. high fluid intake
c. azotemia
d. over hydration
e. pregnancy
f. severe liver disease

C

5

decreased BUN:
a. stress
b. burns
c. high protein diet
d. dehydration
e. severe liver disease (hormones)
f. azotemia

E

6

Clinical application of Urea
A. Evaluation of renal function
B. Assess hydration status
C. Determine nitrogen balance
D. Aid in the diagnosis of renal disease
E. Verify adequacy of dialysis
F. All of the above

F

7

are used most frequently in clinical laboratories for Urea

Enzymatic method

8

The most common method couples the urease reaction with ____________

glutamate dehydrogenase

9

An increase in pH due to the production of ammonia color results in urase (6.8)

Yellow

10

An increase in pH due to the production of ammonia color results in urase (pH 8.2)

bright pink

11

End product of muscle metabolism derived from

creatinine

12

Urea in PFF, the presence of buffer and heat-forming ammonium carbonate which is then reacted with _____ to form a ______

Nessler's reagent, yellow dimercuric ammonium iodide

13

The Oldest method in Urase

Micro-Kjeldahl Nessler

14

Mixture of sulfuric acid and phosphoric acid

Kjeldahl process

15

Coupled Urease

Glutamate Dehydrogenase Method

16

Commonly and Routinely done. Urease is prepared from jack beans. Ammonia treating with Berthelot reagents.

Hydrolysis of Urea by Urease

17

DIRECT MEASUREMENT OF UREA

Diacetyl Monoxime Method (DAM)

18

Diacetyl Monoxime Method (DAM) produces what color

Yellow

19

Principle of Diacetyl Monoxime Method

Faeron Reaction

20

1. _____ will inhibit urase, it should be avoided. 2._______ are added to enhance color development.

1. Fluoride and Citrate
2. Thiosemicarbazide and ferric ions

21

Urea nitrogen concentration expressed in

mg/dL

22

Urea nitrogen concentration converted to urea concentration in ____

mmol/L

23

Specimen used in Urea

plasma, serum, urine, non-hemolyzed

24

Fasting is usually required in Urea and Creatinine. TRUE OR FALSE.

False (not)

25

elevated level of nitrogenous substances like urea and creatinine in the blood

Azotemia

26

very high plasma urea concentration accompanied by renal failure

Uremia

27

conditions in which circulation through the kidneys is less efficient than usual

Pre-renal

28

characterized by the presence of lesions on
the parenchyma itself (tubular injury)

Renal causes

29

due to the obstruction in the-urinary tract due to

Post-renal causes

30

What classification:
Hemorrhage (blood loss)
Cardiac decompression
Increased protein catabolism
Heatstroke (Dehydration)
Burns (Fluid loss)

Pre-renal