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Molecular Biology > Nucleic Acid Extraction > Flashcards

Nucleic Acid Extraction Flashcards

1
Q

Breaking the cell and nuclear membranes.

A

Cell lysis

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2
Q

Nuclei acid extraction in DNA

A

DNA inorganic - salts
Organic - phenol, chloroform
Solid phase - magnetic beads, spin columns

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3
Q

Nuclei acid extraction in RNA

A

Inorganic - guanidine isothiocyanate
Organic - phenol, chloroform
Solid phase - magnetic beads, spin columns

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4
Q

First isolated DNA from human cells

A

Friedrich miescher

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5
Q

Demonstrated the semi-conservative replication of DNA using density gradient centrifugation

A

Matthew meselson and Franklin William stahl

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6
Q

Is a common approach to isolate and purify cell structures for biochemical experiments

A

Density Gradient ultracentrifugation

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Molecular Biology (12 decks)

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