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Molecular Biology > Very Much > Flashcards

Very Much Flashcards

1
Q

Inhibitors of DNA polymerase

A

nitrate, crystals, hemoglobin, and beta-human chorionic gonadotropin

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2
Q

critical for monitoring reagents for carry-over contamina- tion.

A

Reagent blank or contamination controls

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3
Q

aimed at a target that is always present can be incorporated into an amplification assay.

A

amplification control

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4
Q

are amplification controls that mon- itor particular steps of an amplification method

A

Internal controls

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5
Q

FDA-Approved/Cleared Test Methods

Methicillin Resistant Staphylococcus aureus

A

Real-time PCR

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6
Q

Mycoplasma pneumoniae

A

16S rDNA
P1 adhesion gene

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7
Q

Chlamydophila pneumoniae

A

Cloned Pst I fragment
16S rRNA
MOMP

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8
Q

Legionella

A

5S rRNA mip gene

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9
Q

Bordetella pertussis

A

IS 481

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10
Q

Streptococcus pneumoniae

A

lytA (autolysin)
pbp2b

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11
Q

Mycobacterium tuberculosis

A

16S rRNA

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12
Q

test takes 6.5–8 hours to complete

A

AMPLICOR MTB

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13
Q

Treponema pallidum

A

polA

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14
Q

Mycoplasma genitalium

A

rDNA gene

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15
Q

Ureaplasma urealyticum

A

Urease gene

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16
Q

Haemophilus ducreyi

A

groEL gene
p27

17
Q

Neisseria gonorrhoeae

A

opa gene

18
Q

Chlamydia trachomatis

A

MOMP

19
Q

Inhibits protein synthesis

A

Macrolides (erythromycin)

20
Q

Staphylococcus aureus

A

Oxacillin - mecA

21
Q

Streptococcus pneumoniae

A

Penicillin - pbp1a and pbp1b

22
Q

Enterococcus

A

Vancomycin - vanA

23
Q

Salmonella

A

Quinolones - gyrA

24
Q

Mycobacterium
tuberculosis

A

Rifampin - rpoB

25
Q

This method has been used for typing numerous species, including strains of Pseudomonas aeruginosa, Mycobacterium avium, Escherichia coli, N. gonorrhoeae, VRE, and MRSA.

A

Pulsed Field Gel Electrophoresis

26
Q

Criteria for PFGE Pattern Interpretation

Closely related
‘

A

1 2–3. Test isolate is closely related to the outbreak strain

27
Q

This method is performed by cutting DNA with restriction enzymes, resolving the resulting frag- ments by gel electrophoresis, and then transferring the separated fragments to a membrane for probing with a specific probe.

A

Restriction Fragment Length Polymorphism Analysis

28
Q

Performance Comparison of Representative Molecular Epidemiology Methods

Plasmid analysis

A

Good -> Good -> Good -> High -> Good

29
Q

Performance Comparison of Representative Molecular Epidemiology Methods

PFGE

A

High -> High -> High -> Moderate -> Good-Moderate

30
Q

Performance Comparison of Representative Molecular Epidemiology Methods

Chromosomal RFLP

A

High -> Good -> Good -> High -> Moderate-poor

31
Q

Performance Comparison of Representative Molecular Epidemiology Method

PCR-RF LP

A

Good -> Moderate -> Good -> High -> High

32
Q

Performance Comparison of Representative Molecular Epidemiology Method

Sequencing

A

High -> High -> High -> Moderate -> Good-High

Molecular Biology (12 decks)

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