Very Much

Question 1

Inhibitors of DNA polymerase

Answer 1

nitrate, crystals, hemoglobin, and beta-human chorionic gonadotropin

Question 2

critical for monitoring reagents for carry-over contamina- tion.

Answer 2

Reagent blank or contamination controls

Question 3

aimed at a target that is always present can be incorporated into an amplification assay.

Answer 3

amplification control

Question 4

are amplification controls that mon- itor particular steps of an amplification method

Answer 4

Internal controls

Question 5

FDA-Approved/Cleared Test Methods

Methicillin Resistant Staphylococcus aureus

Answer 5

Real-time PCR

Question 6

Mycoplasma pneumoniae

Answer 6

16S rDNA
P1 adhesion gene

Question 7

Chlamydophila pneumoniae

Answer 7

Cloned Pst I fragment
16S rRNA
MOMP

Question 8

Legionella

Answer 8

5S rRNA mip gene

Question 9

Bordetella pertussis

Answer 9

IS 481

Question 10

Streptococcus pneumoniae

Answer 10

lytA (autolysin)
pbp2b

Question 11

Mycobacterium tuberculosis

Answer 11

16S rRNA

Question 12

test takes 6.5–8 hours to complete

Answer 12

AMPLICOR MTB

Question 13

Treponema pallidum

Answer 13

polA

Question 14

Mycoplasma genitalium

Answer 14

rDNA gene

Question 15

Ureaplasma urealyticum

Answer 15

Urease gene

Question 16

Haemophilus ducreyi

Answer 16

groEL gene
p27

Question 17

Neisseria gonorrhoeae

Answer 17

opa gene

Question 18

Chlamydia trachomatis

Answer 18

MOMP

Question 19

Inhibits protein synthesis

Answer 19

Macrolides (erythromycin)

Question 20

Staphylococcus aureus

Answer 20

Oxacillin - mecA

Question 21

Streptococcus pneumoniae

Answer 21

Penicillin - pbp1a and pbp1b

Question 22

Enterococcus

Answer 22

Vancomycin - vanA

Question 23

Salmonella

Answer 23

Quinolones - gyrA

Question 24

Mycobacterium
tuberculosis

Answer 24

Rifampin - rpoB

Question 25

This method has been used for typing numerous species, including strains of Pseudomonas aeruginosa, Mycobacterium avium, Escherichia coli, N. gonorrhoeae, VRE, and MRSA.

Answer 25

Pulsed Field Gel Electrophoresis

Question 26

Criteria for PFGE Pattern Interpretation Closely related '

Answer 26

1 2–3. Test isolate is closely related to the outbreak strain

Question 27

This method is performed by cutting DNA with restriction enzymes, resolving the resulting frag- ments by gel electrophoresis, and then transferring the separated fragments to a membrane for probing with a specific probe.

Answer 27

Restriction Fragment Length Polymorphism Analysis

Question 28

Performance Comparison of Representative Molecular Epidemiology Methods Plasmid analysis

Answer 28

Good -> Good -> Good -> High -> Good

Question 29

Performance Comparison of Representative Molecular Epidemiology Methods PFGE

Answer 29

High -> High -> High -> Moderate -> Good-Moderate

Question 30

Performance Comparison of Representative Molecular Epidemiology Methods Chromosomal RFLP

Answer 30

High -> Good -> Good -> High -> Moderate-poor

Question 31

Performance Comparison of Representative Molecular Epidemiology Method PCR-RF LP

Answer 31

Good -> Moderate -> Good -> High -> High

Question 32

Performance Comparison of Representative Molecular Epidemiology Method Sequencing

Answer 32

High -> High -> High -> Moderate -> Good-High