Nucleic acids Flashcards

(47 cards)

1
Q

What does adenine bond with?

A

thymine

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2
Q

What does guanine bond with?

A

cytosine

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3
Q

What causes the complimentary base pairings?

A

size of the nucleotides

hydrogen bonding between bases

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4
Q

What does the number of purines equal?

A

number of pyrimidines

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5
Q

What are purines?

A

adenine

guanine

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6
Q

How many rings so purines have?

A

double ringed

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7
Q

What are pyrimidines?

A

thymine

cytosine

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8
Q

How many rings do pyrimidines have?

A

single ring

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9
Q

When does the replication of DNA occur?

A

S phase of cell cycle interphase

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10
Q

How does DNA replicate?

A

-The enzyme helicase unzips the DNA (breaks hydrogen bonds)
-SSB (single stranded binging) proteins bind to DNA strands
- DNA polymerase (an enzyme) makes new DNA from deoxyribonucleotides
-Primase makes the primer that tells polymerase where to work
Ligase repairs any breaks in the sugar phosphate backbone

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11
Q

What direction can DNA polymerase work?

A

5’ (prime) to 3’ (prime)

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12
Q

What is the strand that goes in the 5’ to 3’ direction called?

A

Leading strand

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13
Q

What is the strand that goes from 3’ to 5’ called?

A

lagging strand

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14
Q

Why is the lagging strand slow?

A

Because polymers has to put down more primers

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15
Q

What are Okazaki fragments?

A

Fragments of synthesized DNA on lagging strand

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16
Q

What are primers made of?

A

RNA

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17
Q

Why is DNA replication known as semi-conservetive?

A

Because each new DNA contains one old strand and one new one

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18
Q

What is a gene?

A

The segment of DNA that specifies a specific amino acid sequence

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19
Q

How is a gene stored?

A

in the form of a nucleotide acid

20
Q

What does a difference in base sequence cause?

A

A difference in protein structure

21
Q

How do genes control protein synthesis?

A

Not directly

first pass genetic information to RNA

22
Q

How many types of RNA are involved in protein synthesis?

23
Q

What are RNA’s 4 possible nitrogen bases?

A

adenine
guanine
cytosine
uracil

24
Q

What does adenine pair with in RNA

25
What is RNA composed of?
phosphate sugar-ribose nitogen base
26
What are the 3 major classes of RNA
mRNA rRNA tRNA
27
What does mRNA do?
carries genetic information from DNA to ribosomes
28
What does rRNA do?
Site of protein assembly
29
What composed ribosomes?
rRna
30
What happens in transcription?
RNA polymerase connects complimentary RNA bases to the DNA to form mRNA
31
What are the two steps of protein synthesis?
Transcription | Translation
32
Where does transcription takes place?
Nucleus (because that's where DNA is)
33
What happens to mRNA after it is formed?
Leaves the nucleus | goes through the cytoplasms where it attaches to a ribosome
34
What do tRNAs hold
amino acids
35
What is the monomer for a protein?
amino acids
36
What controls what tRNAs (and therefore what amino acids are transferred) come to the ribosome
mRNA
37
What are tRNA's looking for
complimentary bases on mRNA
38
What happened when tRNA find a complimentary base pairing on the mRNA
It transfers the amino acids`
39
What is a codon
A sequence of 3 nucleotides which form a unit of genetic code
40
What contains an anti-codon?
tRNA
41
What are the steps of translation?
Chain initiation chain elongation chain termination
42
What are the causes of gene mutation?
errors in replication mutagens transposons
43
What are mutagens?
environmental influences
44
What are transposons?
genes that move to a new location and disrupt sequences
45
What is DNA's function in the cell
control cell activities
46
What is RNA's function in the cell?
Facilitates protein synthesis
47
What is recombinant DNA (rDNA)?
When DNA from more than one source is isolated and recombined