(O) 31: Mycology I Flashcards

(29 cards)

1
Q

Mycology

A

The study of fungus

fungi are all EUKARYOTIC organisms

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2
Q

Are fungi closer to humans or plants?

A

Fungi ate closer to humans than plants

Most fungi are obligate aerobes (like humans)
- take in oxygen and respire CO2

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3
Q

Amillaria

A

one of largest fungi in the world

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4
Q

Plant cell vs fungal cell

A

Plant
- have cell wall made of cellulose
- have one nucleus per cell
- autotrophs (make their own energy)
- membranes have phytosterols
- store food as STARCH in granules

Fungus
- have cell wall made of CHITIN (crunchy exterior, more rigid than cellulose)
- may be uninucleated or multinucleated
- heterotrophs (need energy from env)
- membranes have ergosterol
- store food as GLYCOGEN in granules

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5
Q

Classification of Fungi

A
  • spore formation
  • fungal genomics
  • modes of nutrition (saprophytic, parasitic, symbiotic)
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6
Q

Mode of Nutrition
Saprophytic

A

fungi obtain their nutrition by feeding on DEAD ORGANIC substances

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7
Q

Mode of Nutrition
Parasitic

A

fungi obtain nutrition by living on other LIVING ORGANISMS and absorb nutrients from their host

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8
Q

Mode of Nutrition
Symbiotic

A

fungi live by having an INTERDEPENDENT relationship w/ other species in which BOTH are mutually benefited

ex. lichens (algae), mycorrhiza (plants)

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9
Q

Fungal Morophology

A
  1. Filamentous fungi
    - macrofungi (ex. mushroom)
    - microfungi (mold)
  2. Yeast
    - microfungi
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10
Q

Filamentous fungi

A
  • fungi grow as filaments called HYPHAE (extend only at extreme tips)
  • fungi exhibit APICAL GROWTH in contrast to may other filamentous organisms
  • fungal hyphae branch repeatedly to make a network called a MYCELIUM
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11
Q

Yeast

A
  • 1% of fungi grow as single-celled yeasts (ex. Saccharomyces cerevisiae)
  • some species are dimorphic and can switch btwn yeast + hyphal (filamentous) phase in response to environmental conditions (pseudohyphae)
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12
Q

Fungal Reproduction

A
  • asexual
  • sexual
  • parasexual
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13
Q

Asexual fungal reproduction

A
  • most COMMON mode
  • asexually reproducing fungi are called ANAMORPHS
  • asexual propagules are called SPORES/CONIDIA produced after mitosis
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14
Q

Sexual fungal reproduction

A
  • sexually reproducing fungu are called TELEOMORPHS
  • sexual propagules are produced by fusion of 2 nuclei that then undergo meiosis
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15
Q

Parasexual fungal reproduction

A

involves genetic recombination without requirement of specific sexual structures

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16
Q

Saccharomyces cerevisiae life cycle

A

Yeast

  1. Asexual reproduction (mitosis) w/ an “a” and an “alpha” cell
    - undergoes budding
  2. Sexual reproduction
    - budding yeast becomes Schmoo cells which then differentiate into zygote
    - zygote then buds and splits into diploids (alpha and “a”)
    - ascus formaton meiosis (2 alpha and 2 “a” cells)
17
Q

Aspergillus Nidulans life cycle

A

Filamentous fungi

Sexual + asexual + parasexual cycles

Asexual
- conidium (seed/spore) lands in soil + germinates to form hyphae
- conidiophores form = more conidia form

Sexual
- 2 nuclei from conidium fuse via meiosis (diploid –> 4 –> 8) to form ascopore
- can do SELF sexual fertilization to make fruiting bodies with ascospores which make hyphae

Parasexual (unstable, no meiosis)
- hyphal fusion to make heterodikaryon
- heterodikaryon undergoes nuclear fusion to make DILPOID HYPHAE (2n)
- chromosome loss to make haploid again

18
Q

Sporulation

A

Biological
- dissemination of spores
- reproduction
- allow fungus to move to new food source (or find more suitable env)
- allows fungus to survive periods of adversity
- introduce new genetic combinations into a population

Practical
- rapid identification (classification)
- source of inocula for human infection and contamination

19
Q

Fungi: friend or foe?

A

Mostly friendly

3% of known mushroom varieties are poisonous to humans

20
Q

Benefits of fungi

A
  • nutrient cycling (frees up nutrients for plants b/c they are decomposers, makes N available + mobilizes Phos.)
  • carbon cycling + climate regulation
  • nutrition and food security
  • human health
  • environmental protection (used to clean up oil spills + get rid of plastics)
  • sustainable materials (mycelium can be used for fabrics)
21
Q

Fungi as food

A
  • mushrooms have been used for food, medicinal purposes as hallucinogenic agents in rituals, or to start a fire for centuries

Asia is biggest producer of mushrooms + truffles

22
Q

Fungiculture and nutrition

A
  • mushrooms are nutritionally LOW IN ENERGY content
  • rich in dietary fibre from vitamin B
  • mushrooms collected outdoors are rich in vitamin D (can also become rich in vitamin D by treatment w/ UV light)

We usually harvest fruitbody

23
Q

Yeast in food production

A

For
- baking
- making alcohol beverages

Yeast fermentation = releases CO2 + produces ethanol

24
Q

Yeast and baking

A

Most common yeast in breadmaking is SACCHAROMYCES CEREVISIAE

  • feeds on sugars in bread dough and produces CO2 gas
  • forms bubbles in dough = expands
  • sugar and eggs SPEED up fermentation
  • fats and salt SLOW fermentation down
  • ethanol is evaporated when bread bakes
25
Yeast and brewing
- yeasts ferment the sugars present in malted barley to produce alcohol - Saccharomyces cerevisiae is used to make ALE-type beers aka TOP-fermenting yeast - bottom-fermenting yeasts like Saccharomyces pastorianus are used to make LAGERS - ethanol tolerance of yeast ranges from 5-21% depending on yeast strain and conditions of env. (yeast self limits)
26
Yeast and wine-making
- alcohol in wine is formed by fermentation of sugars in grape juice w/ CO2 as a byproduct - Saccharomyces cerevisiae is added to ensure fermentation is reliable - sparkling wine is made by adding yeast to wine when it is BOTTLED (CO2 formed in second fermentation is trapped as bubbles)
27
Cheese
Filamentous fungi are important in manufacturing and ripening of: - blue-veined cheeses - soft-ripened cheeses (Brie, Camembert) some cheeses have mold introduced to create a protective rind - others are spiked w/ stainless steel rods to infuse model deep into the cheese
28
Quorn
- mycoprotein developed due to prediction of protein-rch food shortage in 1960s - produced from cultures of fungus FUSARIUM VENENATUM (filamentous fungus) - sold both as a cooking ingredient + as a meat substitute
29
Quorn Production
1. fermentation - to grow organism 2. RNA reduction 3. Centrifuge - separate solids + liquids 4. Chiller - harvest mycoprotein paste 5. Despatch