Oxidative phosphorylation

Question 1

What are some examples of oxidation reactions?

Answer 1

Add O2 to the molecule - (very rare in metabolism)
Remove an electron
Remove a pair of hydrogen atoms

Question 2

What are some examples of hydrogen acceptor cofactors?

Answer 2

NAD+, FAD, NADP+

Question 3

Where does oxidative phosphorylation occur?

Answer 3

In the inner compartments of the mitochondria, mainly in inner mitochondrial membrane

Question 4

What parts of the electron chain are H pair acceptors?

Answer 4

Flavin cofactors

Coenzyme Q

Question 5

What parts of electron chain are electron acceptors?

Answer 5

Iron sulphur proteins

Cytochrome proteins

Question 6

What is an oxido-reduction potential?

Answer 6

The ability of a carrier to donate its electrons to another electron acceptor molecule
Electrons flow from a carrier with a -E0 value to one with a more +E0 value

Question 7

What is the order of oxi-red carriers in the electron transport chain?

Answer 7

NAD→flavin→CoQ→CYT b→cyt C→cyt A→O2

so travels from lowest oxi-reduction potential to highest

Question 8

Whta does the movement of protons do to the membrane?

Answer 8

Cause H+ conc gradient and a voltage gradient across inner membrane

Question 9

What are the inhibitors and uncouplers of oxidative phosphorylation?

Answer 9

Inhib: CN-, rotenone and CO
Uncoup: dinitrophenol (DNP) and thermogenin protein in brown adipose tissue

Question 10

What stages does ATP come from?

Answer 10

2 - glycolysis
5 - pyruvate dehydrogense
20 - TCA cycle
5 or 3 - glycolysis (NADH)