Protein Synthesis

Question 1

Wghat are the 3 steps of transcription?

Answer 1

Initiation - RNA polymerase is direced to start site of transcription on DNA, binds to sigma factor which is boun to Pribnow box on DNA
Elongation - Reading of DNA sequence on template and synthesis of mRNA
Termination - Reaching transcriptional termination site, mRNA synthesis finishes

Question 2

What a stop sequence area look like?

Answer 2

It’s a GC rich area that causes the RNA transcript to form a hairpin loop

Question 3

What is capping?

Answer 3

Formation of methylguanosine cap at 5’ end, GTP reacts with 5’ RNA end

Question 4

What is polyadenylation?

Answer 4

Adds adenosine residues to end of mRNA