P14 - Gel Electrophoresis

Question 1

What Is Gel Electrophoresis Used For?

Answer 1

Separate out the DNA fragments, according to their length.

Question 2

Describe The Process Of Gel Electrophoresis
(9 Points)

Answer 2

~ Make agarose gel.

~ Place gel in a electrolyte solution to conduct electricity.

~ Pipette the DNA samples into wells.

~ Connect the electrodes.

~ DNA is negatively charged, so moves towards the anode.

~ Smaller fragments move faster and further than larger ones.

~ Apply single-stranded DNA probes, complementary to target sequences.

~ Use fluorescent dye to detect bands.

~ Compare band patterns.