P9 - Investigating Antimicrobial Properties Of Plants

Question 1

Describe The Process Of Investigating Antimicrobial Properties Of Plants
(5 Points)

Answer 1

~ Transfer bacteria from broth onto an agar plate.

~ Make sure bacteria are evenly spread out.

~ Prepare plant extracts, by drying and grinding each plant, then soak them in ethanol.

~ Dip absorbent paper into plant extracts and create a control disk.

~ Space disks out evenly on agar plate, tape lid and incubate at 25°C for 24 to 48 hours.

Question 2

Describe The Results That May Incur
(2 Points)

Answer 2

~ Larger the clear zone, more effective the antimicrobial properties of that plant was.

~ Repeat experiment and calculate mean.

Question 3

What Is Meant By Clear Zone?

Answer 3

Area around the disc, where bacteria cannot grow.