pack 1

Question 1

What does the brca mutation do?

Answer 1

Increase breast cancer risk

Question 2

What does the HER2-positive mutation do?

Answer 2

poor response to chemo

Question 3

What are restriction endonucleases?

Answer 3

Endonucleases are isolated from bacteria to be used by scientiscts

Dna is cleaved into smaller fragments by restriction enzymes

They cleave from 4 to 8 nucleotides

these are called restriction sites

Question 4

What are palindromes?

Answer 4

Top and bottom strands have the same exact sequence when read from 5’ to 3’

Question 5

What is an application of DNA cloning?

Answer 5

Human insulin production

Question 6

How does DNA cloning take place?

Answer 6

A plasmid that has antibiotic resistance is cleaved by restriction endonucleases

the DNA of intrest is also cleaved

DNA ligase is used to combine the cleaved plasmid with the cleaved DNA

The recombinant DNA (Hybrid plasmid) is insterted into the bacteria

The bacteria is then placed in a culture with antibiotics to purge any bacteria that doesn’t contain the hybrid plasmid

After the bacteria containing the hybrid plasmid multiplies, it is placed in a tube an killed to extract the multiplied DNA.

The hybrid plasmids are then cleaved again to release the cloned DNA.