pack 3 Flashcards
(6 cards)
1
Q
What is agarose % in electrophioses?
A
It is the concentration of the agarose gell used in the test
The higher the %, the harder it is for the molecules to swim, so the test results will be messy
2
Q
How is electrophoresis set up?
A
Like a horse race
The comb is to create wells to act as the “Staples” for the DNA
3
Q
What dye is used to see the results of electropheoresis?
A
Ethidium bromide
4
Q
What type of gel do you use to get very specfic results about DNA?
A
polyacrylamide
5
Q
What are the different methods used for electrophoresis and what are the gels and types of substances used to test it?
A
6
Q
What are the disadvantges of polyacrylamide?
A
Acrylamide is a neurotoxin
Polyacrylamide is very fragile
