pack 2

Question 1

What are probes and what is its function?

Answer 1

A short piece of complimentary DNA or RNA

Recoginzes a DNA sequence and binds to it

it changes color/emits light when it binds (Molecular beacon)

Question 2

Why aren’t probes used anymore?

Answer 2

Bc they break extremly easily to the presnce of RNAses

Question 3

How does the three step process of probes take place?

Answer 3

dioxygenin or biotin are added, they bind to the dna of interest

Anti-dioxygenin or avidin is added which then binds to dioxygenin/biotin

Lastly, an enzyme is added, antidioxygenin: Horseradish peroxidase or alkaline phosphate

Biotin –> avidin

Question 4

What is the defention of the FISH probes test?

Answer 4

The test uses probes to detect the amplification or deletion of certain genes

Flurosence in situ hybridiazation

Question 5

What is the FISH test usually used to detect and give me an example?

Answer 5

Usually Used to detect STDs

Example (Not releated to STDs):
HER2 gene for breast cancer

Bc there are multiple red dots, it is amplified

Question 6

What is dot blot test and how is it done?

Answer 6

it is a test that uses probes to detect a mutation to know if a patient is a carrier, normal, or infected

There are 2 papers, 1 for normal alele and the other for mutated alele

The papers tell you whether or not there is a mutated/normal alelle based on which one reacted