Past questions 2

Question 1

What kind of theories of origin are there?

Answer 1

A.A. Cell degeneraation

A.B. Cell autonomy

Question 2

Which of the following is direct virus detection?

Answer 2

virus isolation: EM, IF, NA hybridisation, HA

Question 3

Where do you inject Blue tongue virus?

Answer 3

Intravenously into embryonated hen egg

Question 4

Which area of the embryonate egg do we use for haemagglutination test?

Answer 4

Allantoic fluid

Question 5

What does a ‘medium’ contain?

Answer 5

Salts, amino acids, carbohydrates, indicator, antibiotics and antimimetikum

Question 6

What is Trypsin-EDTA used for?

Answer 6

Digestion between the cells of in vitro tissue culture

Question 7

Are there cancer cell cell cultures?

Answer 7

Yes, aneuploid cell culture

Question 8

What is a buffy coat?

Answer 8

White blood cell culture

Question 9

Virus extraction methods?

Answer 9

A. Freezing-thawing three times
B. Ultrasound
C. Detergent

Question 10

With what is virus concentration by precipitation possible?

Answer 10

Ammonium sulphate, PEG, alcohol

Question 11

Are there definitely lipids in virions?

Answer 11

Definitely not, because they are found in the external layer/envelope

Question 12

Are quasi helical viruses always enveloped?

Answer 12

Yes

Question 13

Are icosahedron viruses enveloped?

Answer 13

No

Question 14

What is characteristic of complex viruses?

Answer 14

No capsomere, complete symmetry (pox virus)

Question 15

Pleomorph viruses have no nucleocapsid

Answer 15

True

Question 16

What can we label Nucleic acid hybridisation samples with?

Answer 16

Isotopes or enzymes

Question 17

What can we show with PCR?

Answer 17

Nucleic acids

Question 18

What is needed for sequencing?

Answer 18

Primer, template, deoxynucleotides and marked dideoxynucleotides

Question 19

What cells are formed with the fusion of myeloma and B-cells?

Answer 19

Tetraploid hybrid cells

Question 20

Non-structural proteins do not integrate into the virion

Answer 20

True

Question 21

What is budding?

Answer 21

A type of enveloped virus release

Question 22

Are mutations only a disadvantage?

Answer 22

No, they can be advantageous

Question 23

Can the external environment influence mutation?

Answer 23

Yes

Question 24

Where are there more mutations?

Answer 24

there are more in RNA viruses

Question 25

With mutation, can species-specificity change?

Answer 25

Yes

Question 26

Are clutters thanks to/as a result of Interleukins?

Answer 26

No

Question 27

Is autointerference autoimmune?

Answer 27

No

Question 28

What is the reception of structural proteins

Answer 28

Mixing of phenotypes

Question 29

Do two attenuated vaccines activate each other?

Answer 29

Yes

Question 30

Does intramolecular recombination only happen in DNA viruses?

Answer 30

No

Question 31

Can intramolecular recombination happen in vacuoles and cytoplasm

Answer 31

Yes

Question 32

What causes cell cracking?

Answer 32

Depolarisation of the cytoskeleton

Question 33

How can you send samples?

Answer 33

Via courier, with exact legible details, +4°C, exact address

Question 34

What vectors spread rabbit haemorrhage fever?

Answer 34

Mosquitoes

Question 35

What infects bees?

Answer 35

A. Dicistroviridae (Aparavirus: acute paralysis, acute Israeli paralysis, Kashmar paralysis, Cripavirus: black mother-pearl virus) D. Iflaviridae family (larvae cystic and wing deformity virus)

Question 36

In horses, what causes encephalitis?

Answer 36

A. Togaviridae - alphavirus genus: Eastern, Western, Venezuelan horse encephalitis, Getah virus E. Flaviviridae - flavivirus genus: Japanese encephalitis, St. Louis encephalitis F. Paramyxoviridae + Paramyxovirinae - Henipavirus: Hendra virus G. Bornaviridae - Borneo virus: Bornean sickness

Question 37

All Bunyaviridae, are arbo viruses

Answer 37

False

Question 38

After taking samples, the samples have to be sent to the lab within how many hours?

Answer 38

Usually 24 hours (leukocytes: 6 hours)

Question 39

When are swab samples used?

Answer 39

With body fluid sample

Question 40

Does Border disease cause abortion in sheep?

Answer 40

Yes

Question 41

What form does herpes latency appear as in the nucleus?

Answer 41

Episome (ganglion, gland, macrophage, lymphocyte)