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Flashcards in Questions lab 3 Deck (13)
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1
Q

By PCR

a) we can detect viral antigens in the sample
b) we can amplify virus specific DNA fragments in the sample
c) we can extract nucleic acid from the sample

A

b) we can amplify virus specific DNA fragments in the sample

2
Q
  1. By PCR we amplify
    a) DNA-fragments
    b) RNA-fragments
    c) protein-fragments
A

a) DNA-fragments

3
Q
  1. Which enzyme is used to eliminate the viral proteins from the sample during nucleic acid investigation
    a) trypsine
    b) restriction endonucleases
    c) proteinase
A

c) proteinase

4
Q
  1. The polymerase enzyme copies
    a) the complete DNA sequence
    b) specific segment of the DNA
    c) the complete RNA sequence
A

b) specific segment of the DNA

5
Q
  1. Which ingredients are needed for the PCR
    a) polymerase-I, dideoxy-nucleotides, template DNA, primers
    b) restriction enzyme, phenol, RNA, mononucleotides
    c) Taq polimerase, dezoxy-nucleotides, template DNA, primers
A

c) Taq polimerase, dezoxy-nucleotides, template DNA, primers

6
Q
  1. PCR is done in
    a) thermomixer
    b) thermocycler
    c) cyclometer
A

b) thermocycler

7
Q
  1. During denaturation
    a) the DNA strands get separated
    b) the primers get off the DNA
    c) the enzyme finishes the DNA-sequence
A

a) the DNA strands get separated

8
Q
  1. To transform RNA to DNA we use
    a) Taq-polymerase
    b) RNase
    c) reverse transcriptase
A

c) reverse transcriptase

9
Q

What cleans the DNA from the filter?

A

AE buffer

10
Q

The selectivity of PCR results from?

A

Primers

11
Q

The temperature graph for PCR would look?

A

Cyclical

12
Q

What happens in annealing step?

A

Primers bind to the DNA

13
Q

Taqpolymerase has an optimal temperature of..?

A

72 degrees C