What should be included in the accompanying letter regarding the animal?
Identification number/name of the individual animal, age, sex, brief relevant vaccination history
In case of direct method, at what point should the sample be taken?
In early phase
In case of indirect method, what can be used as samples?
Coagulated blood or serum, milk, liquor, ventricle and organ secretion
A calf has signs of mediastinal pneumonia, what kind of sample is suitable for laboratory investigation?
lung and mediastinal lymphnodes + liver + spleen + kidney
With what can we digest cells used to produce primary monolayer cell culture?
What is contact inhibition?
Division of the cells is continued until cytoplasmic membranes of neighboring cells come in contact with each other
What do we mean by “passage” ?
sub culturing process, producing a secondary cell culture
How fast do you have to send the sample to the laboratory?
within 24h, except leukocyte separation from EDTA blood must be started no later than 6 hours after sample taking
When is the absorption method used to clean the cell cultures?
when the cells are toxic, dead
What do we add to the organ sample to homogenize it?
sterile quartz sand
Why do we use transillumination before inoculation of eggs?
to check the embryonic development, localization of embryo, veins and air sac
At what point should the allantoic cavity be inoculated?
What sample should be taken from a dog that dies from bloody diarrhea?
intestine & mesentherial lymphnoes; (lung, liver, spleen, kidney)
When should the second sample in the paired sera investigation be taken?
2-4 weeks after onset of clinical signs
At what point should the yolk sac be inoculated?