PCR Flashcards

1
Q

What is the purpose of P.C.R?

A

Allows a quantity of DNA to be amplifyied for analysis.

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2
Q

What does P.C.R stand for?

A

Polymerase Chain Reaction

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3
Q

What is the first stage of P.C.R and what happens?

A

Denaturation: The DNA sample is heated to 95 degrees to separate the double stranded DNA into two single strands

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4
Q

What is the second stage of P.C.R and what happens?

A

This stage is know as Annealing. This cools down the strand 50-60 degrees allowing primers (6-26 Bp long) to bind to complementary sequences of each DNA strand.

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5
Q

What is a primer?

A

Short sequence of DNA that flanks the target region

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6
Q

What is the third stage of P.C.R?

A

Extension: The sample is heated to 70 degrees. Thermally Stable Polymarase (TAQ) are added, which increase length of DNA to 56Bps long by making bonds in the sugar phosphate.

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6
Q

What is the final stage of P.C.R?

A

Repeat: The process is repeated 40 times, resulting in an exponential increase in the target sequence.

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7
Q

Name the stages of P.C.R in order

A
  1. Denaturation
  2. Annealing
  3. Extension
  4. Repeat
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