Pg 1-10 Flashcards
(27 cards)
What is Histology?
study of biological materials microscopic structure and functional relations
What does histology allow in relation to comparisons?
Compare healthy vs diseased/abnormal structures
What are forming tissues?
Cells with similar characteristics
What are the 4 tissues?
Epithelial, Muscle, Nerve, Connective
Some cells are adaptable to what 2 types of stress?
Internal & External
What is important to remember about a cell?
Have several functions and be part of more than one cell group
What is a tissue?
Cells arranged in an organised fashion
Organ definition
distinct group of tissues that perform a specific function
Progression of relationship starting with Cells…
Cells -> Tissues -> Organ -> System
What is Cytology?
Analysis of fine cell structure by light microscopy
What technique shows specific DNA and RNA sequences?
In situ hybridization
What is systems Histology?
Study of tissue arrangement at a microscopic level
Pathology definition
Understanding disease processes
Main technique used in histology?
Light Micro + Wax
Range of LM res?
0.2μm - 0.6μm (wax)
2 ways of prepping a slide for observation?
Wax + Freezing
What is histochemical staining?
E.g.?
specific chem reaction between stain and tissue to see certain components
PAS
Enzyme histochemical techniques locate?
Sites of particular enzyme activity
High res light mic can be used with…
Tissue embedded in resin
How is a bone examined histologically?
Decalcifying in acid then sections can be produced
How does an electron microscope differ from a light microscope?
EM uses parallel beams of electrons instead of light waves
What type of microscopy allows resolution of subcellular structures in very thin tissue sections?
Transmission Electron Mic
TEM Summary?
Electrons through fixed tissue and either absorbed or pass through onto a photographic detection plate.
Speed is key
Scanning Elec Microscopy Summary?
3D surface and subcellular views
Dried then coated in Gold and scanned with electron beam (radiation illumination)
