physc/chm methods (2)

Question 1

give moist heat examples

Answer 1

• Pasteurization
• Boiling
• Fractional sterilization
• Autoclave

Question 2

Method of destroying disease producing
organisms in milk and milk products as well as
other beverages.

Answer 2

Pasteurization

Question 3

This method involves utilizing water at boiling temperature of 100 °C.

Answer 3

Boiling

Question 4

Also known as intermittent sterilization and
involves exposing the material to be sterilized to
live steam at 100 °C for 30–90 minutes for
three consecutive days, depending on the
material to be sterilized.

Answer 4

Fractional sterilization

Question 5

Most efficient method of sterilization because it
can destroy all microbial forms.

Answer 5

Autoclave

Question 6

Give examples of Dry heat

Answer 6

• Red flame
• Open flame
• Incineration
• Hot air over
• Infrared rays

Question 7

Used to sterilize articles like bacteriological
wire loops, straight wires, tips of forceps,
and searing spatulas. Uses the Bunsen burner or alcohol lamp

Answer 7

Red flame

Question 8

This method is aimed at burning the organism into ashes. The contaminated material is burned using an incinerator

Answer 8

Incineration

Question 8

Also makes use of the Bunsen burner and alcohol lamp. The material to be sterilized is passed
over the flame several times but is not
heated to redness

Answer 8

Open flame

Question 9

The use of this type of dry heat was introduced by Pasteur where the articles to be sterilized are placed in the oven with a temperature of 160 C. for period of time

Answer 9

Hot air oven

Question 10

In this method, the items to be sterilized are placed in a conveyor belt and passed through a tunnel that is heated by
infrared radiators.

Answer 10

Infrared rays

Question 11

A form of mechanical sieving that does not kill microorganisms but merely separates them from the fluid.

Answer 11

Filtration

Question 12

A _______ is a high efficiency pleated air filter capable of capturing
extremely small particulate matter down to particles that are the size of a
micron (µ), or a micrometer, which is 1/1000th of a meter

Answer 12

High-Efficiency Particulate Air (HEPA) Filter

Question 13

These are more efficient then HEPA Filters. This filter can remove 99.999% of contaminants with diameters of 0.12 µm or larger.

Answer 13

Ultra-Low Particulate Air (ULPA) Filter