PLANT TISSUE CULTURE LAB

Question 1

Growth of protoplasts, cells, tissues, shoot tips, roots, anthers, embryos, flowers and meristems in a laboratory medium.

Answer 1

Tissue culture

Question 2

any nutrient material prepared for the growth and cultivation plant tissue.

Answer 2

culture medium

Question 3

process of making any material entirely free from living microorganism.

Answer 3

sterilization

Question 4

condition that describes the freedom of plant materials, culture medium, confines of the culture vessel from contaminating microorganisms.

Answer 4

asepsis

Question 5

subjecting of cultures to conditions favorable to the growth of the plant tissue

Answer 5

incubation

Question 6

flow air currents in which air currents do not intermingle

Answer 6

laminar flow

Question 7

(CEILINGS) Ceilings shall be at least ______ from the finished floor line.

Answer 7

2.4 m

Question 8

(WALL) All wall tops and ledges shall slope at ______.

Answer 8

45 degree

Question 9

(WALL) It should be coved to the floor-wall and at wall-to-wall junctions with a radius of __________.

Answer 9

50 mm - 60 mm

Question 10

(WINDOWS) All windows shall be properly installed with _________.

Answer 10

16-mesh screen

Question 11

(WINDOWS) Windowsill shall be at least _______ from the finish floor line.

Answer 11

1 m

Question 12

(DOORS) Main doorways shall be at least _______ wide to allow easier movement of large equipment and facilities.

Answer 12

1.5 m

Question 13

(DOORS) Door for staff, storage and chemical rooms should be at least ________.

Answer 13

0.8 m

Question 14

(FLOOR) The floor slope should be ________ towards the drain.

Answer 14

2% - 4%

Question 15

(MEDIA PREPERATION AND STERILIZATION AREA) The area shall be provided with working tables. Tables should be up to ______ wide if
it is to be access from both sides, and should not exceed _______ if it is accessible only from one side and the height should be ________.

Answer 15

1.2 m , 600 mm , 850 mm – 900 mm

Question 16

(GROWTH ROOM) Ceilings shall be insulated with a minimum R-value of ________.

Answer 16

2.64

Question 17

(LIGHTING) Artificial lighting should be provided for the production of photo-period sensitive plants. For the workers, _______ should be provided.

Answer 17

200 lux

Question 18

(GROWTH ROOM) The width of the shelves should be _________ if accessible only from one side and should be _________ if accessible from both sides. The distance between each layer of the shelves shall be _________.

Answer 18

405 mm , 1 m , 455 mm

Question 19

(GROWTH ROOM) Aisles and passage shall be at least ________.

Answer 19

1 m

Question 20

(LIGHTING) Outside the laboratory.

Answer 20

100 lux

Question 21

(LIGHTING) General

Answer 21

200 lux

Question 22

(LIGHTING) Working table

Answer 22

500 lux

Question 23

(LIGHTING) Growth room

Answer 23

100 - 1000 lux