post lab 1-3 Flashcards
(150 cards)
1
Q
It supports the tube and connects it to the base
A
Arm
2
Q
The bottom of the microscope, used for support
A
Base
3
Q
It is where you look to see the image of your specimen
A
Eyepiece
4
Q
Instrument used to see objects that are too small to the naked eye
A
Microscope
5
Q
It connects the eyepiece to the objective lenses
A
Body tube
6
Q
This is the part that holds two or more objective than says and can be rotated easily change power
A
Revolving nose piece
7
Q
The flat platform where you place your slide
A
Stage
8
Q
Small, round knob of the side of the microscope used to fine-tune the focus of your specimen
A
fine adjustment knob
9
Q
This is usually used specifically using HPO
A
Fine adjustment knob
10
Q
Large, round knob on the side of microscope used for focusing the specimen
A
coarse adjustment knob
11
Q
Holds the slide in place
A
Stage clips
12
Q
It is the hole in the stage through which the base light reaches the stage
A
Aperture
13
Q
It is used to call it and focus the light from the illuminator onto the specimen
A
Condenser
14
Q
It is located under the stage often in conjunction with an iris diaphragm
A
Condenser
15
Q
It controls the light going thourpugh aperture
A
iris diaphragm
16
Q
It is used to reflect light to the specimen for source of light
A
Mirror or light source
17
Q
It is used to increase the magnification of the specimen
A
Objective lenses
18
Q
Total magnification of scanning
A
40
19
Q
Total magnification of LPO
A
100
20
Q
Total magnification of HPO
A
400
21
Q
Total magnification of OIO
A
1000
22
Q
Magnification lenses of 40 times
A
High power objective
23
Q
Magnification of scanning
A
Four times
24
Q
Magnification lens of LPO
A
10
25
Magnification length of white
100
26
t or f: Always Observe the specimen or object using the high power object first
false, low power
27
It is to bring the object into focus
Coarse adjustment knob
28
Bring the object into sharp focus
Fine adjustment knob
29
t or f: Use only the final adjustment knob when using the highest power objective
true
30
Keep one eyes open to reduce eyestrain
False, both eyes
31
Keep eye slightly below the eyepiece to reduce eyelash interference
false, above
32
To find out the total magnification of the object, multiplied the power of the eyepiece lens by the power of the objective
true
33
t or f: Always use one hand to move the microscope
false, both hands
34
t or f: Place one hand around the arm and then put your other hand under the base of the scope for support
True
35
t or f: One nucleus in one sense except for WBC and platelets
false: RBC and platelets
36
t or f: Muscle cells are non-nucleated
false: Multi nucleated
37
Type of cells that contains a nucleus and organelles
Eukaryotic cell
38
A type of cell that does not include the nucleus or specialized organelles
Prokaryotic cell
39
It make up the living structure of the body
Somatic cell
40
These are germ cells that involved in sexual reproduction
Sex cells
41
It is a double membrane bound control center of the cell
Nucleus
42
These are hereditary units
genes
43
It is a nuclear structure that has double membrane that separates the nucleus from the cytoplasm
Nuclear envelope
44
It is a nuclear structure that has numerous openings in the nuclear envelope
Nuclear pores
45
It is a nuclear structures that control the movement of the substance between nucleus and cytoplasm
nuclear pore
46
It is a nuclear structure that is a small discrete spherical densely staining structures made up of RNA and produces ribosomes
nucleolus
47
These are long molecules of the Deegan is that combines with protein molecules
Chromosomes
48
protein molecules in the DNA
Histones
49
It is very small rounded bodies found on both ends of the nucleus
Centrosome
50
It regulates the rate of cell division and multiplication
Centrosome
51
It consist of cylinder with a microtubules arrange peripherally in a circle
Centrioles
52
Two types of hereditary traits
Phenotype and genotype
53
these are physical type of traits that is observable aspects of the hereditary handed down by parents of the offspring
Phenotype
54
This type of trait is non-physical and non-observable
Genotype
55
Center of chromosomes
Centromere
56
It is the arrangement of molecule with in the membrane that resembles the sea of lipids
Fluid Mosaic model
57
These are the gatekeepers to certain molecules and ions
protein
58
Organelle that separates the cells internal environment from the outside Environment
Plasma membrane
59
Composition of plasma membrane
Carbohydrates proteins and lipids
60
It is a small is spherical sac formed by budding off from a membrane
Vesicle
61
Materials move into a sale in a vesicle formed from the plasma membrane
Endocytosis
62
Vesicles fuse with the plasma membrane’s, releasing their contents into the extracellular fluid
Exocytosis
63
It is a transport of the combination of endocytosis and exocytosis
Transcytosis
64
It is an intracellular fluid that surrounds the organelles And a marker of lactate the hydrogenase
cytosol
65
1 g of carbohydrates is equal to how many calories
4 cal
66
Heat insulator and tissue elasticity
Fat or lipids
67
Fat or lipids are stored in
Triglyceride
68
It is for tissue building
Protein
69
Cytoplasm composition
Carbohydrates, fats or lipids, proteins, electrolyte
70
Most abundant composition of cytoplasm
Water
71
Two types of water in cytoplasm
Fix water and free state water
72
water composition in males
55 to 65%
73
Water composition of females
45 to 55%
74
Water composition in infants
70 to 80%
75
It is a network of fibers extending throughout the cytoplasm
Cytoskeleton
76
Also forms the major component of cilia and flagella
Cytoskeleton
77
Cytoskeleton consist of
Microfilaments, microtubules and Intermediate filament
78
it is a part of cytoskeleton that helps to cell change and maintain its shape
Microfilament
79
It is a part of cytoskeleton that often used by the cells to hold their shape
Microtubules
80
Site for proteins synthesis
Ribosomes
81
It translates the genetic code into polypeptide chains
Ribosomes
82
It is found attached to the Rough endoplasmic reticulum or free in the cytoplasm
Ribosomes
83
It is a process where amino acid join together by the peptide bond or polypeptide chains
Protein synthesis
84
Composition of ribosome
60% RNA 40% protein
85
These are specialized and metabolically active structures or little organs within the cells
Organelles
86
It’s transport of materials within the cell
Endoplasmic reticulum
87
It is a network of continuous sacs, studded with ribosomes
Rough endoplasmic reticulum
88
It manufactures, process and transport the proteins for export from the cell
Rough endoplasmic reticulum
89
It involved in the synthesis of lipids, carbohydrate metabolism and detoxification of drugs and poisons
SER
90
Golgi complex is consist of how many flattened membranous sacs
3 to 20
91
it’s temporary store depot for cellular secretion and site of synthesis of large carbohydrates
Golgi complex
92
It is an intracellular defense mechanism that will digest the substance digested by the phagocytosis
Lysosome
93
It is a single membrane bound structure that contains digestive enzyme
Lysosome
94
It does not engage in any digestive activity and newly formed lysosomes
Primary lysosomes
95
Sites of current or past digestive activity
Lysosomes secondary
96
It is where the production and degree Dacian of hydrogen peroxide occurs
Peroxisome
97
These are the series of folds in the mitochondria
cristae
98
These are the large central fluid filled cavity of mitochondria
Matrix
99
These are the cytoplasmic inclusion
Vacuoles, inclusion, pigment and fat droplets
100
It is a color compound that says my produce oranges
Pigments
101
Color of the bilirubin
Yellow
102
Color of biliverdin
green
103
It is the process by which we give color to a section
Staining
104
It is used to identify a differentiate bacteria
Gram stain
105
Gram-positive color
Purple or violet
106
Gram-negative color
Red or pink
107
Bacteria in gram positive
staphylococci and streptococci
108
Bacteria in gram negative
neisseria
109
Components of giemsa stain
Methylene blue and eosin
110
It is used to identify acid-fast bacilli
ziehl nielsen stain
111
primary stain in hot sputum
carbol fuschin
112
primary stain in cold tissue
carbol fuschin
113
secondary stain in cold tissue
malachite green
114
secondary stain in hot sputum
methylene blue
115
hot sputum is by
ziehl nielsen
116
cold tissue by
kinyoun
117
steps in gram staining ( in order)
crystal violet, iodine, alcohol and safranin
118
mordant in hot sputum
physical (steam)
119
mordant in cold tissue
chemical (tergitol)
120
decolorizer in hot sputum
0.05 or 0.1 N hcl acid in 70% ethanol
121
decolorizer in cold tissue
0.05 or 0.1 N hcl acid in 70% ethanol
122
color of non acid fast decolorizer
colorless
123
color of non acid fast secondary staining
blue/green
124
causative agent of tuberculosis and leprosy
Mycobacterium
125
Used to identify myelin which stains blue
luxol fast blue
126
Used to identify Other elements of the nervous system which is stains pink or violet
cresyl violet
127
This is stain mainly used to evaluate the type and amount of extracellular material like collagen, fibrin, muscle and elastic fibers
trichrome stain
128
It’s principle is smaller dye molecules will penetrate and stain a tissue element
Masson’s trichrome stain
129
Collagen fibers stains
masson’s trichrome stain and van gieson
130
elastic fibers stain
verhoeff’s stain, orcein stain, weigert’s fuschin stain, aldehyde fuschin
131
reticular fibers stain
gordon & sweet’s stain and gomori’s stain
132
muscle stain
mallory ptah,heidenhain’s iron hematoxylin and masson’s trichrome
133
carbohydrates stain
periodic acid schiff, alcian blue, mucicarmine, colloidal iron stain
134
amyloid stain
CRYSTAL VIOLET
CONGO RED
SIRIUS RED
THIOFLAVIN -t
135
lipid stain
OIL RED O
SUDAN BLACK B
OSMIUM TETROXIDE
136
nerve cell stain
NISSL STAIN
BIELSCHEWSKY STAIN
EAGER’S STAIN
137
BONE stain
SCHMOR’S PICROTHIONINE
GOLDNER’S TRICHROME
SOLOCHROME
138
melanin stain
MASSON FONTANA SILVER STAIN
SCHMORL’S FERRICYANIDE
139
It is a genetic disorder which increase iron absorption and deposition in various organs
Hemochromatosis
140
It is a versatile dye that colors nucle blue
toluidine blue
141
in van gieson stain, the color of nuclei is
blue or black
142
in van gieson stain, the color of cytoplasm, muscle, fibrin and RBC is
yellow
143
in VG stain, the color of collagen is
red
144
stain used in the diagnosis of acute lymphocytic leukemia
orcein stain
145
Stain used to identify deposits of protein in tissue
congo red
146
It is a versatile tool that used to distinguish elements of the extracellular matrix
alcian blue
147
It is a histologic dye that is used primarily stain acidic union
Copper phthalocyanine dye
148
It is a dye that is more soluble in fat than in water or alcohols
Oil red O
149
What is the stain which principle is dilute mineral acid Hydrolysis releases ferric iron from protein bound tissue deposits
perl’s prussian blue
150
What is the stain of perls prussian blue
potassium ferric ferrocyanide
