Post Laboratory Yeast Morphology and Reproduction

Question 1

eukaryotes
exist in a unicellular state
few are pathogens
several fermentation processes

Answer 1

yeasts

Question 2

a mat made up of
intertwining thread like
hyphae

Answer 2

true mycelium

Question 3

• the filaments that
  compose the body of the
  fungus

Answer 3

hyphae

Question 4

a number of buds in
succession that remain
joined for a period of
time

Answer 4

pseduomycelium

Question 5

pseudomycelium grows in ____
aerobic conditions

Answer 5

reduced

Question 6

way of reproduction of pseudomycelium

Answer 6

budding

Question 7

true mycelium may give rise to

Answer 7

arthrospore
blastospore

Question 8

to give rise to an
organism(s) which are
genetically identical to
the parent

what reproduction

Answer 8

asexual

Question 9

most yeasts do this

what kind of reproduction

Answer 9

asexual

Question 10

examples of asexual spores

Answer 10

arthrospore
blastospore
chlamydospores

Question 11

results from fusion of
two opposite mating types of the same species

Answer 11

sexual spores

Question 12

sexual spores include

Answer 12

basidiospore
ascospore

Question 13

asexual spores can be reproduced through

Answer 13

budding
fission

Question 14

yeast that is undergoing fission

Answer 14

schizosaccharomyces

Question 15

yeast that is undergoing budding

Answer 15

saccharomyces sp

Question 16

formed
in a sac (ascus)

what kind of sexual spore

Answer 16

ascospore

Question 17

ormed externally on
a pedestal (basidium)

what kind of sexual spore

Answer 17

basidiospore

Question 18

yeast cultures include

Answer 18

Candida parapsilosis
* Debaryomyces hansenii
* Hansenula anomala
* Endomycopsis burtonii
* Pichia fermentans
* Saccharomyces cerevisiae

Question 19

Observe for the presence of TRUE MYCELIA and
PSEUDOMYCELIA

what method

Answer 19

dalmau plate method

Question 20

Observe for colony morphology on PDA and ACA
plates: surface, edge or margin, color and texture

what procedure

Answer 20

cultural characteristics

Question 21

• Observe the shape of the vegetative cell, presence
  of visible internal structures, method of asexual
  reproduction

what procedures

Answer 21

asexual reproduction

Question 22

• Observe ascospores (or basidiospores, if present)

what procedures

Answer 22

method of sexual reproduction

Question 23

Candida parapsilosis

expected results on: dalmau plate method

Answer 23

pseudomycelium

Question 24

Candida parapsilosis

expected results on: cultural characteistics

Answer 24

wrinkeld form
undulate margin
raised elavation
white colored
roguh

Question 25

Candida parapsilosis expected results on: aseuxal reproduciton

Answer 25

budding ovoid, elliptical and elongated

Question 26

Candida parapsilosis expected results on: mode of sexual reproduction

Answer 26

1-4 hat shaped ascospores

Question 27

saccharomyces cerevisiae expected results on: dalmau plate mthod

Answer 27

pseudohyphae (pseudomycelia actual)

Question 28

What is the purpose of the coverslip in Dalmau plate?

Answer 28

Aids in focusing and prevents the objective to be contaminated by inadvertently lowering it into the inoculated agar Provides the partial anaerobiosis condition

Question 29

How is Schizosaccharomyces morphologically different from Saccharomyces? Linear, rod shaped cells Fission as the asexual mode of reproduction

Answer 29

schizosaccharomyces

Question 30

Linear, rod shaped cells Fission as the asexual mode of reproduction Globous or ellipsoidal in shape Budding as the asexual mode of reproduction

Answer 30

saccharomyces

Question 31

Provides nutrient base for luxuriant growth of most fungi and dextrose that serves as a growth stimulant what agar

Answer 31

potato dextrose agar

Question 32

A nitrogen-deficient “starvation” medium what agar

Answer 32

aCA

Question 33

Has low nutrient concentration thereby inducing the spore formation, therefore the cell will become resistant to starvation what media

Answer 33

McConkey Agar

Question 34

Weak binding dye to the cell wall and spore wall what stain for ascospore staining

Answer 34

malachite green

Question 35

Make spore wall more permeable to malachite green Malachite green will attach to the peptidoglycan

Answer 35

steaming

Question 36

Rapid growth in population

Answer 36

asexual reproduction

Question 37

More genetic diversity to allow adaptations

Answer 37

sexual reproduction

Question 38

What other characteristics should be used to accurately identify yeasts?

Answer 38

Biochemical tests Physiological tests Molecular identification

Question 39

early colonies white and velvelty, later becoming green or black, vesicles carrying sterigmata on part of whole vesicle

Answer 39

aspergillus

Question 40

rapid growth, white at first, later becoming green or other colors, velvety or powdery surface, septate hyphae, branching conidiophores and chains of conidia

Answer 40

penicillum

Question 41

rapid growth, thin white cottony, later ecoming green or yellow green and powdery, septate hyphae, short branched conidiophores with clusters of conidia

Answer 41

trichoderma

Question 42

rapid growth, white cottony surface later becoming pink, rose, purple, or yellow in color

Answer 42

fusarium

Question 43

very rapid growth, dense cottony mycelia, first white, later gray or yellowish brown, non septate hyphae, unbranched sporangiophores arising

Answer 43

rhizopus

Question 44

rapid growth dense white fluffy surface later becoming gray or yellowish brown

Answer 44

mucor

Question 45

mucor is septate/non septate fungi

Answer 45

non septate

Question 46

rhizopus is septated/non septated fungi

Answer 46

rhizopus

Question 47

sporangiophores simple: or branched; columella round, cylindrical or pear-shaped; no stolons or rhizoids; spores smooth and regular

Answer 47

mucor

Question 48

rhizoids and stolons present; sporangiophores arise at the node where rhizoids are also formed; sporangia usually large and black; columella hemispherical with cupshapetl apophysis

Answer 48

rhizopus

Question 49

septated fungi include (7)

Answer 49

aspergillus penicillium geotrichum trichoderma cladosporium alternaria fusarium

Question 50

septated, brancheci mycelium usually uncolored; conidiophore or stalk septate arise from foot cell; conidiophore swells, vesicles bearing sterigmata from which the conidia are cut-off; conidia in chains

Answer 50

aspergillus

Question 51

septa tet{ branching mycelia; conidiophores br.anched or unbranched; brushJike spore heacls, sterigmata or phialides borne in clusters anrl essentially in one plane

Answer 51

penicllum

Question 52

yeast-like fungi; growth appear as firm, felFlike mass which later become soft and creamy;septated hyplla, dichotomously branched; arthrosporesrectangular (submerged), oval (aerial)

Answer 52

geotrichum

Question 52

many branched condiophores, final branch being a sterigma which cuts off spherical to slightly ovate, bright green conidia int

Answer 52

trichoderma

Question 53

seplate and dark mycelia; largc' sporeheads, tree-like clusters of dark conidia (buds); dark, ovate, budding conidia- one-celled when young, two-celled when old

Answer 53

cladosporium

Question 54

dirty gary-green, loose, wooly septate mycelium; large, ovate to inverted dubshaped multicellular conidia witjr both cross and lorrgihrdinal or diagonal walls; conidiophore bearing a chairr of ccnidia with the blunt ecige of each conidium towards the mycelium; conidiophores branched or unbranched

Answer 54

alternaria

Question 55

several-celled, slckle-shaped macroconidium; one-celled, ovoid or oblong microconidia usually are also preseng conidia borne singiy or in chains

Answer 55

fusarium

Question 56

teleomorph (perfect or sexual state) of Fusaium; ascigerous form of, sp o ral a ti o n; ch aract niz ed by deep blue or purple p erithecia; fusoid one septate ascospore

Answer 56

fusarium

Question 57

various shape of yeast cell

Answer 57

ovoid elongate cylindrical ellipsoidal subglobose spheroidal apiculate

Question 58

check various sizes of yeast cell

Answer 58

+1

Question 59

type of asci and ascospore

Answer 59

hat shaped spherical reniform saturn shaped

Question 60

filamentous structure arising from mature buds which do not detach from the mother cell

Answer 60

pseudomycelium

Question 61

filamentous structure resulting from continuous growth of the hyphal tip

Answer 61

true mycelium