Practical Exam Essentials Flashcards
are enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes.
biosafety cabinets
biosafety cabinets are provided with ___-filters that decontaminate the air moving out of the cabinet.
HEPA
only provides protection to the sample and not to the personnel and the environment, whereas biosafety cabinets protect all three.
laminar hood
Biosafety cabinets are classified into ___classes by the U.S. Centers for Disease Control and Prevention (CDC), each with specific performance characteristics and application
three
are most suitable for work with hazardous agents that require Biosafety Level 3 or 4.
what BSL level
BSCs III
is the most basic biosafety cabinet that provides protection to the environment and the laboratory personnel.
BSC I
It doesn’t, however, provide protection to the product as the unsterilized room air is drawn over the work surface.
BSC I
Class I biosafety cabinets are typically used to either enclose specific equipment like ___ or for procedures like aerating cultures that might potentially generate aerosols
centrifuges
Biosafety cabinets of this class are either ducted (connected to the building exhaust system) or unducted (recirculating filtered exhaust back into the laboratory).
BSC I
provide both kinds of protection (of the samples and the environment) since makeup air is also HEPA-filtered.
BSC II
The principle of operation of Class II cabinets involves a fan mounted in the top of the cabinet that draws a curtain of ___ ___ over the workstation where the biological products are being handled.
sterile air
are leak-tight, totally enclosed but ventilated cabinets, where all air that either enters or leaves through the facility pass through a HEPA filter.
class III cabinets
The cabinets are provided with rubber gloves that are attached to the system to be used during operations in the cabinet. This is why these cabinets are also termed ‘glove boxes’.
BSC III
is a technique used to identify Candida albicans and other yeasts by observing their morphology, specifically the formation of chlamydospores and pseudohyphae
dalmau plate method
view dalmau plate method results
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which is conidia/sporangiospore in slide 2?
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identify parts of sporangiospore (slide 3)
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identify the parts of a conidiophore (slide 5)
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identify thallus parts slide 7
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identify mycelia type slide 9
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view slide culture technique
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is a semi-synthetic medium used for the general cultivation of fungi, particularly those that utilize sodium nitrate as their sole nitrogen source, and is often used for isolating species like Aspergillus, Penicillium, and Paecilomyces.
czapek dox agar
is a culture medium primarily used for the cultivation and isolation of fungi and yeasts, particularly dermatophytes, due to its acidic pH which inhibits bacterial growth
saboraud dextrose agar
s a widely used microbiological growth medium, particularly for cultivating fungi and yeasts, made from potato infusion and dextrose.
potato dextrose agar
