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Microbiology. INFECTION > Practical > Flashcards

Practical Flashcards

1
Q

Laboratory diagnosis of infectious diseases are 2 methods, name them

A

Direct methods
Indirect methods

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2
Q

Direct methods are

A

Detection of pathogen

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3
Q

Indirect methods are

A

Detection of Abs in patient serum
اخذناها في الاميون

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4
Q

Obtain sample (specimen) according to patient ____

A

complaint
مثل البلغم

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5
Q

Direct methods are 3 types , name them

A

Microscopy

Non-culture methods

Isolation on culture medium tion of colony

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6
Q

In the microscopy method , direct film detect ______

A

Morphology of bacteria

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7
Q

Non-culture methods are 2 ways

A

Nucleic acid detection(e.g pcr)

Ag detection(direct immunofluorescentence)

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8
Q

Isolation on culture medium are 2 steps ,first _____ with its 4 ways
Then ________

A

Identification of colony

Antibiotic susceptibility tests

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9
Q

Identification of colony by

A

Colonial morphology
Microscopic appearance
Motility tests
Biochemical reactions

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10
Q

What is culture media?

A

Artificial substances → Supply bacteria with nutrients → Growth & multiplication

Visible effect

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11
Q

What are Visible effect of bacteria

A

Visible effect
Colony : on solid media
Turbidity: on fluid media

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12
Q

Types of media

A

Enriched
Basic
Selective
Enrichment
Media for anaerobic culture

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13
Q

BASIC MEDIA Constituents

A

Contain minimal nutrients → Growth of bacteria that don’t have special nutritional requirements

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14
Q

Examples for basic media :

A

Nutrient broth
Peptone H20
Nutrient agar

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15
Q

Nutrients broth are

A

Peptone H20 + Meat

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16
Q

Nutrient agar contain

A

Nutrient broth + agar powder

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17
Q

ENRICHED MEDIA
Constituents and Uses–>

A

Contains blood, serum or egg—>
Growth of fastidious organisms (need ↑ nutrients) which can’t grow on basic media

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18
Q

E.g for ENRICHED MEDIA

A

Blood agar
Chocolate agar
Loeffler’s serum

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19
Q

Blood agar is

A

Nutrient agar + blood

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20
Q

Chocolate agar is ______ which
Release _____

A

Heated blood agar

Factor X and V

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21
Q

Loeffler’s serum
Contain

A

Horse serum + glucose broth

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22
Q

Bacteria in Blood agar differentiated according to their _____

A

haemolytic activity

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23
Q

Hemolytic activity of bacteria are

A

Alpha (partial ) haemolysis
Gama(No ) haemolysis
Beta(complete) haemolysis

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24
Q

Selective media contains

A

Contains selective substance: chemicals or antibiotics

25
Q

Selective media uses

A

Inhibits growth of some organisms (mainly flora or commensals) → allows growth of others → isolation of organisms from specimens containing flora e.g stools,throat swab

26
Q

Types of selective media

A

Selective and enriched
Selective
“ and differential

27
Q

selective & Enriched
Are

A

Thayer Martin

Lowenstein Jensen(green fluid)
Blood tellurite

احفظ شكلهم

28
Q

Thayer Martin contain

A

Chocolate agar + Antibiotics

29
Q

Lowenstein Jensen contain

A

Egg + Malachite green

30
Q

Blood tellurite contain

A

Blood agar + K tellurite

31
Q

E.g for selective media selective

A

Sabouraud Dextrose agar

Dextrose سكر العنب

32
Q

Name Culture of fungi agar

A

Sabouraud Dextrose agar

33
Q

In selective media selective:
•______ →- bacteria

•______→ - saprophytic fungi

A

•Chloramphenicol + Acidic pH

Cyeloheximide

34
Q

Selective & Differential
Constituents :

A

Contains selective substance + sugar & indicator (changes in color if pH changes)

35
Q

Selective & Differential Uses :

A

If organism ferments sugar → acidic pH → indicator changes in color

36
Q

E.g for selective and Differential media

A

1-MacConkey ‘s agar

2-Thiosulfate citrate bile sucrose agar(TCBS)

3-Deoxycholate Citrate agar (DCA)

احفظ أشكالهم

37
Q

Selective substance of macConkeys agar

A

1) Bile salts
2) Crystal violet

38
Q

Selective substance of TCBS

A

1) Bile salts
2) Thiosulfate + citrate
3) Alkaline pH

39
Q

MacConkeys are used on with type of bacteria

A

Culture of intestinal Gram -ve bacilli

40
Q

Thiosulfate citrate bile sucrose agar (TCBS) are used on which type of bacteria

A

Culture of Vibrio

41
Q

Suger and indicator of macConkeys is

A

Lactose & Neutral red
(pink transparent)

42
Q

Suger and indicator of TCBS

A

Succrose and Bromothymol blue (Green)

43
Q

Non Lactose fermenter —> MacConkeys agar is

A

Pale yellow colonies

44
Q

Lactose fermenter—>MacConkeys agar is

A

Rose pink colonies

45
Q

Non Succrose fermenter—>TCBS agar is

A

No reaction

46
Q

Succrose fermenter—->tcbs agar is

A

Yellow colonies
احفظ الشكل

47
Q

ENRICHMENT MEDIA
Constituents

A

Fluid Selective → Inhibit growth of intestinal commensals (flora)

48
Q

ENRICHMENT MEDIA Uses

A

→ Preliminary step for isolation of intestinal pathogens

49
Q

ENRICHMENT MEDIA e.g

A

Selenite broth
Tetrathionate broth
Alkaline peptone H2o

لا شكل

50
Q

CULTURE MEDIA FOR ANAEROBE steps

A

A) 1st on Robertson cooked meat—>
Broth + cooked meat (contains hemin & glutathioneتسحب الاوكسجين)—->
Subculture on solid medium ( Nutrient agar Or Blood agar)
—-> Anaerobic environment—->
B) Anaerobic GasPak System

51
Q

BIOCHEMICAL REACTIONS Methods are

A

Conventional Methods

Commercial Modifications of Traditional Biochemical tests

52
Q

I-Conventional Methods are

A

Effect on proteins
Ability to utilize citrate

Effect on proteins &Carbohydrates

Enzymatic tests

53
Q

Enzyme tests are

A

Catalase test
Coagulase test
Oxidase test
Gelatinase test
Urease test

54
Q

Effect on proteins are

A

Indole test
Urease test
Gelatinase test

55
Q

Ability to utilize citrate
E.g

A

Citrate test

56
Q

Effect on proteins & Carbohydrates
E.g

A

Litmus milk
Triple sugar iron (TSI) agar

57
Q

Catalase test mechanism

A

Colony + H2O2—>Catalase—>
H2O+O2 (Effervescence)

احفظ الصورة

58
Q

Oxidase test mechanism

A

Reagent (colorless) turns purple

59
Q

Gelatinase test mechanism

A

Breaking down gelatin (semisolid< 24 C)
Irreversible liquefaction

تذكر الصورة

Microbiology. INFECTION (4 decks)

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