practical

Question 1

kinyon primary stain is

Answer 1

Carbol fuschin

Question 2

flourochrome counter stain is

Answer 2

potassium permanganate

Question 3

what is the appearance of a positive MGIT tube

Answer 3

florescence under UA light

Question 4

1-9 AFB organisms/field on kinyoun stain is:reported as:

Answer 4

+3

Question 5

Effect of room temp >85 C

Answer 5

increase reactivity giving false positive

Question 6

Serum that agglutinates w/ the cardiolipin reagent may be reported as: Treponoman pallidum

Answer 6

reactive or weakly reactive

Question 7

Positive RPR tests should be confirmed w/:

Answer 7

FTA-
EIA
TP-PA
TPHA

Question 8

clinical gram stain quantity should be should be expressed as

Answer 8

few
modarate
many
rare

Question 9

trichrome stain is fixed by

Answer 9

PVA

SAF

Question 10

Acid fast stains are fixed with

Answer 10

SAF

formalin

Question 11

Fluorescent stains are fixed with:

Answer 11

formalin

Question 12

Fixative used for recovery of helminth eggs:

Answer 12

formalin

Question 13

Counterstain in modified acid fast stain is:

Answer 13

Methylene blue

Question 14

Giemsa stain would be appropriate for:

Answer 14

Trypanosoma cruzi, P. falciparum

Question 15

what are the smears used for ID

Answer 15

thin smear

Question 16

what is the color of the background of the trichrome stain

Answer 16

green

Question 17

what is the color of Cytoplasm- trophs in trichrome

Answer 17

blue green

Question 18

what is the color Cytoplasm cysts in trichrome stain

Answer 18

cysts = blue green, may be purple tinged.

Question 19

what is the color if Nuclei in trichrome strain

Answer 19

red, sometimes tinged with purple

Question 20

what is the color of cellular illusion in tricrome stain

Answer 20

red, sometimes tinged with purple

Question 21

Fungal elements on calcafluor will appear:

Answer 21

blue-white or green fluorescent

Question 22

Two microscopic structures associated w/ Candida albicans:

Answer 22

germ tubes

chlamydocanidia

Question 23

Fungal elements on calcafluor will appear:

Answer 23

blue white or green fluorescent

Question 24

In the Cryptococcal Antigen test for CSF, latex particles are bound to what?

Answer 24

Antibodies to the polysaccharide capsule

Question 25

Rapid testing for influenza is usually based on which methodology?

Answer 25

EIA

Question 26

Acid fast stains are typically done to find what organism?

Answer 26

Mycobacteria tuberculosis Norcardia spp Cryptosporidium

Question 27

name 3 examples of Beta-Lactams

Answer 27

``` penicillin, amipicllin, piperacillin , cephazolin, imipenem ```