micro unit 2 Flashcards

(92 cards)

1
Q

Thermally dimorphic

A

When dimorphism is temperature dependent, the fungi are designated as thermally dimorphic

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2
Q

dematiaceous

A

Hyphomycetes are those conidial fungi that produce dark brown, green-black, or black colonies and are the causative agents of phaeohyphomycosis such as Phialophora

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3
Q

fungal filaments are know as

A

Hyphae

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4
Q

A mass of hyphae collectively make a

A

Mycelium

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5
Q

Zygomycete(phylum)

A

fungi with sparsely septate hyphae

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6
Q

Thermally dimorphic fungi temperature range

A

yeast 35-37 C

mold 25-30 C

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7
Q

Hyaline

A

Hyphomycetes include those conidial fungi which colonies may be colourless or brightly coloured. These include the agents of hyalohyphomycosis, aspergillosis, dermatophytosis and the dimorphic pathogens, like Blastomyces

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8
Q

blastoconidia

A

A yeast form which it morphology is defined as single celled that reproduces itself by simply binding to from blastoconidia

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9
Q

conidia

A

Asexual spores. a structure such as mushrooms consist simply of a number of filaments parked tightly together and reproduction by spore or conidia

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10
Q

pseudohyphae

A

consist of elongated cells with constriction at the attachment

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11
Q

chlamydospores

A

/////////

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12
Q

elementary body

A

????????????????elements of the body that protect you from having a fungal infection for example heat

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13
Q

treponemal antibodies

A

This are used to detect if you are positive for syphilis test
Specific antibodies (treponemal) are generated to the spirochete membrane proteins
specific

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14
Q

reaginic antibodies

A

reacts with general test for syphilis

Non-specific

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15
Q

: Inhibitory Mold Agar

A

this is a selective media
Candida albicans
Aspergillus niger
richophyton mentagrophytes

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16
Q

Sabhouroud Dextrose agar- SABHI

A

primary recovery of saprobic and pathogenic fungi

isolates and enhances growth of pathogenic fungi

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17
Q

mold

A

??????

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18
Q

saprohytes

A

like to live on dead organic matter

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19
Q

Brain heart Infusion Agar

A

medium is for bacteria, yeast and pathogenic fungi.

recovery of pathogenic fungi and saprobic

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20
Q

cornmeal agar

A

identification of Candida albicans by chlamydospore production.

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21
Q

Bird niger seed agar

A

identification of Cryptocossus neoformans. It produces a brown pigment

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22
Q

Dermatophyte test media

A

????????

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23
Q

•How does Rhizopus spp*** grow well in which media and how does it apper

A

sab-dex plate

Gray wooly colony

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24
Q

the appearance of Rhizopus spp*** under the microscpe

A

check in the notes

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25
Growth requirements of Rhizopus spp***
24 to 96 hrs 1-5 days
26
what type of fungi is a of Rhizopus spp***
Aseptate hyphae Mucorales thermally Monomorphic mold
27
infections of of Rhizopus spp***
May cause systemic (through out the body)or cutaneous infections (skin infection)
28
specimen of Rhizopus spp***
``` skin, respiratory secretions nose brain , stool wounds ```
29
special growth Rhizopus spp***
????????
30
ID Rhizopus spp***
?????
31
Reservoirs Rhizopus spp***
???????
32
Cryptococcus neoformans** gram stain
gram negative bacilli
33
Cryptococcus neoformans** source of infection reservoirs
weatherd pigeon droppings, bird feces and soil
34
Cryptococcus neoformans**, media
Sabouraud's dextrose agar bird seed agar corn meal agar used microscopic characteristic of yeast
35
Cryptococcus neoformans** appearance on media
appearance on Sabouraud's dextrose agar cream coloured, smooth, moist, shining and mucoid Brown colonis on Bird seed agar
36
Cryptococcus neoformans** growth requirements
?????
37
Cryptococcus neoformans** infectious form and infetion
form yeast | infection Opportunistic Fungi
38
specimen for Cryptococcus neoformans**
CFS, sputum ,pus , blood and biopsy tissue
39
Cryptococcus neoformans** ID
India ink
40
Name a yeast that contains phenoloxidase, produces melanin and has a capsule
Cryptococcus neoformans**
41
Penicillium sp** stain
Lactophenol cotton blue prep
42
Penicillium sp** media
Sab-dex agar and appers blue green velvety to powdery
43
Penicillium sp** microscopically
hyphae are hyaline and septate
44
Penicillium sp** growth requirements
2-6 days 35C
45
*Penicillium sp* unique
its a rapid grower
46
KOH alone is used on what
on skin scraping to see if there are fungal elements
47
*Penicillium sp** infection
Opportunistic Fungi, often produces an allergic reaction
48
Penicillium sp** specimen
respiratory secretions, skin, ear cornea, urine
49
Penicillium sp** ID
Microscopic: septate hyhae with brushlike condiophores.
50
Penicillium sp** reservoirs
?????
51
Aspergillus fumigatus**, stain
??????
52
Aspergillus fumigatus** media
Czapek dox agar and potato dextrose agar
53
Aspergillus fumigatus** growth requirement
temp of up to 45C
54
Aspergillus fumigatus** infection
opportunistic and its a human pathogen
55
Aspergillus fumigatus** specimens
``` skin and nail scrappings sputum and bronchial washings cerebrospinal fluid, pleural fluid and blood tissue biopsies ```
56
Aspergillus fumigatus ID
microscopic morphologic smooth-walled and have conical shaped terminal vesicles, which support a single row of phialides on the upper two thirds of the vesicle
57
Aspergillus fumigatus unique
rapid grower
58
Aspergillus fumigatus reservoirs
soils and decaying organic debris
59
Aspergillus fumigatus is what type of fungi
its a mold septate Hyphae Hyaline
60
Microsporum canis** stain
?????
61
what type of fungi is Microsporum canis***
Mold septate hyphae Hyaline
62
Microsporum canis*** growth requirement
1 week
63
Microsporum canis*** ID
vitro hair perforation test is positive | microscopic ID Thick walled, spindle shaped multiseptate, rough walled microconidia
64
Microsporum canis*** colony
orange yellow
65
Microsporum canis*** infection
children and adults get infected by contact with infected animals
66
Microsporum canis*** unique
vitro hair perforation test is positive | ring worm infection in cats and dogs.
67
Microsporum canis*** direct detection
cofluor white or potassium hydroxide reveals small spores outside the hair
68
Microsporum canis*** reservoirs
cats and dogs
69
Microsporum canis*** specimen
hair
70
Coccidiodes immitis** gram stain
gram pos
71
Coccidiodes immitis** media
Sabouraud's dextrose agar showing a suede-like to downy, greyish white colony with a tan to brown reverse.
72
Coccidiodes immitis** growth
2-21 days
73
Coccidiodes immitis** infection
pulmonary infections, skin infection meningitis arthritis
74
Coccidiodes immitis** specimen
``` respiratory secretions skin bone CFS synovial fluid urine blood ```
75
Coccidiodes immitis** ID bio safety should be
bio safety 3
76
Coccidiodes immitis** is what type of fungi
Mold Septate Hyphea Hyaline Diamorphic mold
77
Coccidiodes immitis** reservoirs
soil inhibiting fungus | found in desert potion
78
Coccidiodes immitis** infection
by inhalation
79
Rickettsia rickettsii gram stain
Small pleomorphic Gram negative rods
80
Rickettsia rickettsii media
???????
81
Rickettsia rickettsii growth requirements
??????
82
Rickettsia rickettsii infections
rocky mount spotted fever
83
Rickettsia rickettsii vectors
ticks
84
Rickettsia rickettsii diagnostic tests
serology,immunohistology, PCR with sequencing
85
Rickettsia rickettsii infections
Produces vascular lesions | Symptoms; fever, headache and rash
86
Rickettsia rickettsii specimens
Infects vascular endothelial cells; typically skin, heart, lung, muscle and brain
87
Chlamydia trachomatis gram stain
gram-negative
88
Chlamydia trachomatis ID or test
nucleic acid amplification test (NAAT) Weil-Felix test; agglutination of Protus vulgaris by serum of infected patients (OX-19, OX-2) and Proteus mirabilis (OX-K) presumptive test. Must confirm with IFA, EIA, CF, and Western blot, or microimmunofluorecence.
89
Mycoplasma pneumonia gram stain
none
90
Mycoplasma pneumonia media
Selective media Selective media PPLO, Biphasic, mod. NYC)
91
Mycoplasma pneumonia Growth requirements
agar and broth 37 C for 4 weeks
92
Mycoplasma pneumonia infection
lower and upper respiratory tract infection, Asymptomatic infection