PRE LAB EXPERIMENT 1

Question 1

Used to contain serum or other body fluids as well as the reagents in order for us to demonstrate a particular reaction.

Answer 1

PLAIN TEST TUBES

Question 2

In serology laboratory, we are after

Answer 2

antigen- antibody reaction

Question 3

Used to contain or transfer different volumes

Answer 3

SEROLOGICAL PIPETTES

Question 4

NOTE: If we want to transfer a 5ml amount of fluid, we should use the pipette having a 5 ml amount of fluid; If we want to transfer 3 mL amount of fluid, it is much better to use (?) pipette instead of 10mL.

Answer 4

5 mL

Question 5

To demonstrate agglutination or flocculation reactions

Answer 5

AGGLUTINATION CARDS/SLIDE (KLINE AGGLUTINATION SLIDE)

Question 6

Example (flocuculationn reactions):

Answer 6

C-reactive protein Test (white- being the flocculation is white)
Antistreptolysin Test

Question 7

specially in the preparation of red cell suspension, it is much better to use a

Answer 7

GRADUATED CENTRIFUGE TUBE

Question 8

There are two types: glass and plastic

Answer 8

GRADUATED CENTRIFUGE TUBE

Question 9

Used as a platform for microscopic specimen observation

Answer 9

GLASS SLIDE (ORDINARY)

Question 10

Two types: glass and plastic

Answer 10

PASTEUR PIPETTE

Question 11

Plastic if more preferred compared to glass

Answer 11

PASTEUR PIPETTE

Question 12

: sensitive to glass Pasteur pipette

Answer 12

(Normal saline Solution)

Question 13

Cloth and rubber

Answer 13

TOURNIQUET

Question 14

used if the patient has a latex allergy or if a patient is hairy esp. in man

Answer 14

Cloth TOURNIQUET

Question 15

disadvantage: the lock is easy to be broken

Answer 15

Cloth TOURNIQUET

Question 16

Lavender:

Answer 16

EDTA

Question 17

Blue:

Answer 17

sodium citrate

Question 18

Gray:

Answer 18

sodium fluoride

Question 19

Green:

Answer 19

heparin

Question 20

: whether it does not contain an anticoagulant OR it contains a clot activator

Answer 20

Red

Question 21

In drawing blood, the bevel upon inserting the needle should be facing you

Answer 21

Syringe

Question 22

: always observe the hub if there is a backflow; pull the plunger slowly because if not, it is prone to hemolysis of the sample

Answer 22

Syringe

Question 23

LANCET • Two types:

Answer 23

small lancet and feather lancet (ouch)

Question 24

Has adjustments in order for you to choose what depth of puncture do you want

Answer 24

AUTOLANCET

Question 25

Malfunctions most of the time

Answer 25

AUTOLANCET

Question 26

NOTE: it is better to use (?) when it comes to capillary blood collection

Answer 26

manual force

Question 27

Disadvantage: you can’t see the backflow of the hub especially if you have stick it already to the patient

Answer 27

2-WAY NEEDLE AND HOLDER/ADAPTER

Question 28

Used to confirm sometimes the agglutination reaction

Answer 28

COMPOUND MICROSCOPE

Question 29

Rotate to agglutination card/ slide to observe properly the agglutination reaction

Answer 29

MECHANICAL ROTATOR

Question 30

For separating plasma and serum from the packed RBC

Answer 30

CLINICAL CENTRIFUGE

Question 31

To demonstrate agglutination especially when using tube tests

Answer 31

CLINICAL CENTRIFUGE

Question 32

Use to sterilize glassware that are being used in serology laboratory

Answer 32

CLINICAL STERILIZER

Question 33

All sections in the lab are just sharing a(CC, CM, Serology)

Answer 33

1 clinical sterilizer

Question 34

Place some tests that require incubation

Answer 34

WATER BATH

Question 35

Same concept with clinical sterilizer

Answer 35

HOT OVEN

Question 36

Used to sterilize glassware

Answer 36

HOT OVEN

Question 37

1000 ul, 500 ul, 10-100 ul (yellow tip)

Answer 37

BLUNT TIP NEEDLE

Question 38

The tips will depend on the capacity of micropipette

Answer 38

BLUNT TIP NEEDLE

Question 39

used in serology laboratory

Answer 39

TUBERCULIN SYRINGE

Question 40

To administer allergen

Answer 40

TUBERCULIN SYRINGE

Question 41

For example: Mantoux test, For testing if you type IV hypersensitivity

Answer 41

TUBERCULIN SYRINGE

Question 42

used for immunoassays

Answer 42

MICROTITER PLATE

Question 43

For example: Enzyme immunoassays - this type of plate is already coated with a reagent

Answer 43

MICROTITER PLATE

Question 44

used to rim the sides of the test tube, also used for mixing

Answer 44

WOODEN APPLICATOR STICK/APPLICATOR STICK

Question 45

either with we put soap, most of the time in serology lab, we are placing here are your NSS (Normal saline solution), to be easier to make red cell suspension

Answer 45

WASH BOTTLE

Question 46

Part of analytical phase of quality assurance; process of monitoring results from control samples to verify accuracy of patient results.

Answer 46

QUALITY CONTROL (QC)

Question 47

Note: is not performed for your equipment but also each process of the laboratory. It is important to verify the accuracy of the patients result that we are releasing.

Answer 47

QUALITY CONTROL (QC)

Question 48

Sample that is chemically & physically similar to unknown specimen & is tested in exactly the same manner.

Answer 48

CONTROL

Question 49

Monitors precision of test system.

Answer 49

CONTROL

Question 50

For nonwaived quantitative tests, CLIA requires at least 2 levels of controls each day test is performed. (See "Equivalent QC" below.)

Answer 50

CONTROL

Question 51

For qualitative tests, pos & neg controls must be included with each run.

Answer 51

CONTROL

Question 52

Before we ran patient sample, make sure that we have ran first the control. Never ever release a sample if you did not yet run the control.

Answer 52

CONTROL

Question 53

Not only applicable in chemistry, but also all the section of the laboratory that will be using a machine.

Answer 53

CONTROL

Question 54

Testing control material not built into test system. Term also used for QC that extends beyond lab, e.g., participation in proficiency testing program.

Answer 54

EXTERNAL QC

Question 55

DOH mandated that all laboratory should participate in

Answer 55

national external quality assurance scheme (NEQAS).

Question 56

Electronic, Internal, or procedural controls that are built into test system.

Answer 56

INTERNAL MONITORING SYSTEMS

Question 57

This is what we practice inside the clinical laboratory.

Answer 57

INTERNAL MONITORING SYSTEMS

Question 58

Under that will also be the different QC will apply for each equipment that is being used.

Answer 58

INTERNAL MONITORING SYSTEMS

Question 59

Microscopes - Clean & adjust

Answer 59

4 times/yr.

Question 60

Temperatures - (?) of incubation, heating blocks, water baths, refrigerators, freezers.

Answer 60

Daily checks

Question 61

Quarterly

Answer 61

Speed Timer

Question 62

Yearly

Answer 62

Function

Question 63

Day of use

Answer 63

Tube fill level (serologic) Temperature check (refrigerated centrifuge)

Question 64

Weekly

Answer 64

Saline fill volume (serologic)

Question 65

Volume of antihuman globulin dispensed (if applicable)

Answer 65

Monthly

Question 66

Check temp daily

Answer 66

Waterbaths Heat blocks

Question 67

Heat blocks check each well.

Answer 67

Periodically

Question 68

Centrifuges Determine optimum speed & time for different procedures upon receipt - after repairs -

Answer 68

QUARTERLY PERIODICALLY

Question 69

Centrifuges Check timer every

Answer 69

3 mos

Question 70

Cell washers Check tube fill level

Answer 70

Daily

Question 71

Cell washers Verify time & speed.

Answer 71

quarterly

Question 72

Pipettes Calibrate.

Answer 72

quarterly

Question 73

Labs may reduce frequency of testing external OC materials for certain test systems if they can demonstrate through a CMS-approved QC evaluation process that test system is stable.

Answer 73

EQUIVALENT QC

Question 74

Here in the Philippines, we are not yet performing

Answer 74

EQUIVALENT QC

Question 75

Centrifuges RPM every (with tachometer)

Answer 75

6 mos

Question 76

Cell washers AHG volume.

Answer 76

monthly

Question 77

Temperature verification (refrigerated centrifuge)

Answer 77

Monthly