Principles Of Immunodiagnostics Flashcards by Jhaunte Braithwaite

Q

What are the factors affecting measurement of antigen-antibody interactions?

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Q

Contrast affinity and avidity

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Q

What are the useful properties of monoclonal antibodies?

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Q

What are the useful properties of polyclonal antibodies?

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Q

What are immunoassays?

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Q

Explain the principle behind precipitation

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Q

What are the precipitation assays?

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Q

What are the factors affecting measurement of antigen-antibody reactions?

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Q

What is immunoelectrophoresis?

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Q

What are agglutination reactions?

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What is direct agglutination?

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Q

What is passive agglutination?

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Q

What’s the principle behind hem agglutination?

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Q

What is hemagglutination inhibition?

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Q

What are the components of hemagglutination and hemagglutination inhibition ?

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Q

What are the limitations of agglutination?

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Q

Summarize ABO antibodies

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Explain blood typing

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Q

Summarize blood type, blood antibodies and antigens

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Q

What does blood type look like?

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What are immunoflourescent Assays?

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Q

Contrast direct and indirect immunofluorescent assays

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Q

What does immunofluorescent assays look like for antibodies ?

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Q

What are the limitations of immunofluorescence?

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What is the principle of Enzyme-linked immunosorbant assay?

What are the types of ELISA?

What is the principle of western-blot analysis?

What does western blotting look like?

What are the advantages and limitations of western blot?

What are the main indirect methods of immunochromatography?

What is immune-chromatography?

What are the advantages and disadvantages of indirect methods of immuno-chromatography?

What is flow cytometry?

When do you use Ag vs Ab test?

What’s the impact of Sero-conversion?

Describe the generations of immunoassays and seroconversion

explain timing of antibody response in natural infection