PROTEINS

Question 1

ZONE ELECTROPHORESIS SEPARATES PROTEINS BASED OF pH.

pH GRADIENT IS ESTABLISHED BY THE INCORPORATION OF SMALL POLYANIONS AND POLYCATIONS IN THE GEL.

IT USES POLYACRYLAMIDE OR AGAROSE GEL MEDIUM

Answer 1

ISOELECTRIC FOCUSING

Question 2

CONDITIONS IN WHICH AMYLOID PROTEINS ARE ABNORMALLY DEPOSITED IN ORGANS/TUSSUES

Answer 2

AMYLOIDOSIS

Question 3

ALBUMIN BINDS TO DYE, SPECIFIC, SENSITIVE & PRECISE

Answer 3

BROMOCRESOL PURPLE

Question 4

FORMATION OF A FINE PEPTIDE WHICH SCATTERS INCIDENT LIGHT

Answer 4

NEPHELOMETRY

Question 5

LOW MOLECULAR WEIGHT PROTEIN AND CYSTEINE PROTEINASE INHIBITOR

ENDOGENOUS RENAL MARKER OWING FOR ITS SENSITIVITY

FREELY FILTERED BY GLOMERULUS AND COMPLETELY REABSORBED

Answer 5

CYSTATIN C

Question 6

IT BINDS IN LIPIDS AND PROTEINS

IT TRANSPORTS CHOLESTEROL AND TAG AND PHOSPHOLIPIDS

Answer 6

LIPOPROTEIN

Question 7

BIOCHEMICAL MARKER OF BONE RESORPTION THAT CAN BE DETECTED IN SERUM AND URINE

MOST USEFUL FOR MONITORING THE RESPONSE TO ANTIRESORPTIVE THERAPY

Answer 7

CROSS-LINKED C-TELOPEPTIDES

Question 8

IT CAUSES HEPATIC CIRRHOSIS

Answer 8

BETA-GAMMA BRIDGING

Question 9

MOST ABUNDANT PROTEIN IN PLASMA AND IT MAINTAINS OSMOTIC PRESSURE

Answer 9

ALBUMIN

Question 10

MEMBER OF PENTRAXIN PROTEIN FAMILY

IS A CARDIAC MARKER USED AS AN EARLY WARNIG TEST TO PERSON AT RISK OF CORONARY ARTERY DISEAS

Answer 10

C-REACTIVE PROTEIN

Question 11

BALANCE BETWEEN ANABOLISM AND CATABOLISM

Answer 11

NITROGEN BALANCE

Question 12

MOST COMMON USED IN HIGH RESOLUTION PROTEIN ELECTROPHORESIS

Answer 12

AGAROSE GEL

Question 13

used to detect malnutrition and individual’s response to dietary supplementation.

Answer 13

PREALBUMIN

Question 14

Role: copper-binding glycoprotein that has enzymatic activities.

Answer 14

CERULOPLASMIN

Question 15

estimates low albumin levels; most commonly used dye.

Answer 15

BROMOCRESOL GREEN

Question 16

largest major nonimmunoglobulin protein in plasma.

Answer 16

a2-MACROGLOBULIN

Question 17

exhibits affinity with vitamin D and actin.

Answer 17

GROUP SPECIFIC COMPONENT GLOBULIN (GC-GLOBULIN)

Question 18

most widely used method and the one recommended for the determination of total protein.

Answer 18

BIURET METHOD

Question 19

USED AS TUMOR MARKER

Answer 19

ALPHA-FETO PROTEIN (AFP)

Question 20

MARKER FOR CONGESTIVE HEART FAILURE

Answer 20

B-TYPE NATRIURETIC PEPTIDE (BNP)

Question 20

diagnostic tool in neonates with bacterial infections.

Answer 20

a1-ACID GLYCOPROTEIN

Question 21

binds and inactivates prostate-specific antigen.

Answer 21

a1-ANTICHYMOTRYPSIN

Question 22

TRANSPORTS IRON TO ITS STORAGE SITE

Answer 22

TRANSFERRIN

Question 23

GLOBULINS CAN BE SEPARATED FROM ALBUMIN BY SALTING-OUT

Answer 23

SALT FRACTIONATION

Question 24

CONDITIONS DETECTED BY SERUM AFP (2)

Answer 24

NEURAL TUBE DEFECTS DOWN SYNDROME

Question 25

WHAT IS ELEVATED OR INCREASE IN NEPHROTIC SYNDROME

Answer 25

a2-MACROGLOBULIN

Question 26

presence of two albumin bands instead of a single band in electrophoresis.

Answer 26

BISALBUMINEMIA

Question 27

is an inborn error of metabolism characterized by the absence of homogentisate oxidase in the tyrosine pathway. DARKENING OF URINE UPON STANDING

Answer 27

ALKAPTONURIA

Question 28

is an autosomal recessive trait characterized by the deficiency of the enzyme phenylalanine hydrolase

Answer 28

PHENYLKETONURIA

Question 29

REFERENCE INTERVAL FOR SERUM TOTAL PROTEIN

Answer 29

6.5-8.3 g/dl

Question 30

usually measured by subtracting the value of serum albumin from the total protein concentration.

Answer 30

GLOBULIN

Question 31

OTHER NAME FOR PREALBUMIN

Answer 31

TRANSTHYRETIN

Question 32

binds free hemoglobin by its alpha-chain.

Answer 32

HAPTOGLOBIN

Question 33

binds and inactivates prostate-specific antigen

Answer 33

a1-ANTICHYMOTRYPSIN

Question 34

It is a small heme protein found in skeletal and cardiac muscles. It transports and stores oxygen from hemoglobin to intracellular respiratory enzymes of contractile cells. It has higher affinity for oxygen than does hemoglobin.

Answer 34

MYOGLOBIN

Question 35

is characterized by markedly reduced or absence of a-ketoacid decarboxylase. It results to accumulation of branched-chain amino acids

Answer 35

MAPLE SYRUP URINE DISEASE

Question 36

most significant clinical application of SPE is for the identification of monoclonal spike of immunoglobulins

Answer 36

SERUM PROTEIN ELECTROPHORESIS (SPE)