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LIPIDS METHODOLOGY Flashcards

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1
Q

FIRST STEP AFTER THE INTAKE OF FOOD OR LARGE FAT DROPLET

A

EMULSIFICATION USING BILE

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2
Q

BILE IS SYNTHESIZED BY THE

A

LIVER

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3
Q

PRIMARY CONTENT OF BILE

A

CHOLESTEROL

BILIRUBIN

BILE SALTS

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4
Q

VERY SMALL AGGREGATES OF TRIGLYCERIDES IN CHOLESTEROL

A

MICELLES

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5
Q

HYDROLYZE/ CONVERT TRIGLYCERIDES INTO FATTY ACIDS AND MONOGLYCERIDES

A

PANCREATIC LIPASE

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6
Q

TRIGLYCERIDES ARE COMPOSED OF (2)

A

1 GLYCEROL

3 MOLECULES OF FATTY ACIDS

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7
Q

FATTY ACIDS ARE COMPOSED OF (2)

A

FATTY ACIDS

MONOGLYCERIDE

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8
Q

CHYLOMICRONS ARE FORMING INSIDE OF THIS

A

GOLGI APPARATUS

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9
Q

SITE OF SYNTHESIS OF CHYLOMICRONS

A

INTESTINES

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10
Q

CHYLOMICRON IS RICH IN WHAT APOLIPOPROTEIN AND IT IS THE INDICATOR

A

APO B48

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11
Q

90% is
10% is

A

90% TRIGLYCERIDES
10% CHOLESTEROL

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12
Q

CHYLOMICRON REMNANT IS RICH IN AND THE ACTIVATOR OF REMNANT

A

APO E

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13
Q

INTERMEDIATE DENSITY LIPOPROTEIN IS RICH IN _______ & SEEN INCREASED IN ________ or ______

A

APO E

HYPERLIPOPROTEINEMIA OR DYSBETALIPOPROTEINEMIA

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14
Q

LOW DENSITY LIPOPROTEIN IS RICH IN_______ AND CARRIES_______FROM______ TO______

A

APO B100

CHOLESTEROL

LIVER TO PERIPHERAL TISSUES

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15
Q

MOST INTEREST IN DIAGNOSIS OF MANAGEMENT OF LIPOPROTEIN DISORDERS (4)

A

ATERIOSCLEROSIS (THICKENING OF ARTERIES)

OBESITY

HYPERTENSION

DIABETES MELLITUS

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16
Q

EXAMPLE OF ATERIOSCLEROSIS IS

A

ATHEROSCLEROSIS (PLAQUE OR THICKENING OF LIPIDS)

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17
Q

INDICATIONS FOR LIPID PROFILE (3)

A

SCREENING FOR PRIMARY AND SECONDARY HYPERLIPIDEMIAS

MONITORING RISK OF ATHEROSCLEROSIS

MONITORING TREATMENTOF HYPERLIPIDEMIAS OR DYSBETALIPIDEMIAS

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18
Q

PREANALYTICAL CONSIDERATIONS (5)

A

AGE (SCREENED IN THE AGE OF 20 Y/O

SEX (WOMEN HAVE LOWER LEVEL THAN MEN EXCEPT IN CHILDHOOD AND EARLY 50s

SEASON

FOOD INTAKE

MEDICAL CONDITIONS (PATIENTS WITH HYPERTHYROIDISM HAS DECREASED CHOLESTEROL AND TAG & PATIENTS WITH HYPOTHYROIDISM HAS INCREASED CHOLESTEROL AND TAG)

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19
Q

PATIENT FAST FOR ____HRS BEFORE SAMPLING FOR _______

A

12 HOURS

LIPID PROFILE ONLY

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20
Q

PATIENT FAST FOR ____ BEFORE SAMPLING IN _______

A

10 HOURS

FBS AND LIPID PROFILE

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21
Q

CHYLOMICRONS ARE CLEARED WITHIN _____ AFTER _____ HRS FAST

A

6-9 HOURS

12 HOURS

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22
Q

FLOATING CREAMY LAYER IN THE SAMPLE

A

CHYLE

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23
Q

INABILITY TO CLEAR CHYLOMICRON AND THE ACTIVATOR OF LIPOPROTEIN LIPASE

A

APO CII

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24
Q

POSTURE DECREASES _____
PATIENT TO BE SEATED FOR _____
PROLONGED VENOUS OCCLUSION LEADS IN CHOLESTEROL INCREASE CONCENTRATION BY _____

A

10%

5 MINUTES

10-15%

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25
MILD EXERCISE DECREASE IN CONCENTRATION OF _____ AND ______
CHOLESTEROL TRIGLYCERIDES
26
WHO WALKED FOR 4 HOURS CHOLESTEROL IS ____% LOWER AND HDL IS ____HIGHER
5% 3.4%
27
MENSTRUAL CYCLE CHOLESTEROL AND TAG CONCENTRATION HIGHEST AT _______
MIDCYCLE
28
HIGH FAT DIET INCREASES ________ PLASMA TAG IS REDUCED WHEN _______ IS REDUCED
SERUM TRIGLYCERIDES SUCROSE
29
ALCOHOL INGESTION SERUM TAG IS INCREASED MORE THAN ______
20mg/dl
30
CETP MEANS AND FUNCTION
CHOLESTEROL ESTER TRANSFER PROTEIN HIGH CETP LOW HDL
31
MOST PREFERRED SAMPLE
SERUM PLASMA IN EDTA TUBE
32
2 MOST IMPORTANT METHOD OF LIPOPROTEIN DETERMINATION
ULTRACENTRIFUGE ( REFERENCE MERHOD FOR LIPOPROTEIN ELECTROPHORETIC METHODS
33
SERUM CAN BE USED TO STORE SAMPLES IN WEEK FOR_____& MONTHS FOR_____
WEEKS FOR -20C MONTHS FOR -70C
34
PREFERRED ANTICOAGULANT
EDTA
35
CONVERSION FACTOR OF SERUM CHOLESTEROL IS
1.03
36
ANTICOAGULANT THAT EXERT LARGE OSMOTIC EFECT
SODIUM CITRATE
37
ANTICOAGULANT THAT CANNOT BE USED IN ELECTROPHORESIS
HEPARIN
38
ANALYZED IN FROZEN SAMPLES (3)
TOTAL CHOLESTEROL TRIGLYCERIDES HDL
39
MEASURED IN FROZEN SAMPLES
APOLIPOPROTEIN
40
FORMER REFERENCE METHOD IN CHEMICAL METHOD
ABELL-KENDALL METHOD
41
METHOD THAT HAS 3 STEPS
ABELL-KENDALL METHOD 3STEPS ARE SAPONIFICATION (USING ETHER) EXTRACTION (USUNG HEXANE) COLORIMETRY
42
REACTION THAT HAS BLUISH GREEN COLOR
LIEBERMANN -BURCHARDT REACTION
43
IT IS A COLOR DEVELOPER
GLACIAL ACETIC ACID
44
METHOD THAT HAS 2 STEPS
BLOORS METHOD 2STEPS EXTRACTION COLORIMETRY
45
END PRODUCT OF LIEBERMANN BURCHARDT REACTION IS CALLED
CHOLESTADIENYLMONOSULFONIC ACID
46
COUNTERPART OF LIEBERMANN BURCHARDT REACTION IS
SALOWSKI METHOD
47
END PRODUCT OF SALOWSKI METHOD
RED COLOR AND CHOLESTADIENYLSULFONIC ACID
48
ASSAY OF CHOICE IN ENZYMATIC METHOD
CHOLESTEROL OXIDASE METHOD
49
TINDERS REACTION OF CHOLESTEROL OXIDASE METHOD
4-AMINOPHENAZONE—PEROXIDASE——QUINONEIMINE DYE
50
DILUTION FACTOR OF CHOLESTEROL OXIDASE METHOD
1:2
51
REFERENCE METHOD AND MEASURES CHOLESTEROL
GC-MS METHOD
52
CONVERSION FACTOR OF CHOLESTEROL
0.026
53
PRECIPITATION METHOD USES ______ AND ______
DIVALENT CATIONS AND POLYANIONS
54
REMOVE THE NON-HDL AND MOST COMMON FOR HDL
POLYANION PRECIPITATION
55
COMPLEXED TO MAGNETIC PARTICLES DOES NOT REQUIRE CENTRIFUGATION USE IN AUTOMATED CC ANALYZER
MAGNETIC METHOD
56
FIRST REAGENT IN HOMOGENOUS ASSAY OR DIRECT HDL ASSAY
BLOCKS NON-HDLs ALSO USED BLANKING STEP
57
REFERENCE METHOD FOR HDL ESTIMATION
THREE STEP PROCEDURE
58
THREE STEP PROCEDURE ______TO REMOVE VLDL HEPARIN____PRECIPITATION USES ________METHOD IT IS TEDIOUS AND EXPENSIVE
ULTRACENTRIFUGATION MANGANESE ABELL KENDALL METHOD
59
ALL CLASSES OF LIPOPROTEIN IN SINGLE RUN AND USED REAGENT
DENSITY GRADIENT METHOD POTASSIUM BROMIDE
60
DENSITY OF POTASSIUM BROMIDE
1.063
61
CALCULATION METHOD USED IN LDL ESTIMATION
FRIDEWALD EQUATION
62
USEFUL IN EVALUATION OF TYPE III HYPERLIPOPROTEINEMIA
VLDL OR PLASMA TRIGLYCERIDE RATIO
63
FORMER REFERENCE METHOD OF TRIGLYCERIDES
VAN HANDEL AND ZILVERSMITH METHOD
64
END COLOR OF TAG
PINK COLOR
65
APOLIPOPROTEIN AN INDICATOR OF COMBINED LDL AND VLDL CONCENTRATION
APO B
66
MAJOR PROTEIN OF HDL
APO A-1
67
MOST COMMON METHOD AND EASILY ADAPTED SPECTROPHOTOMETRIC ANALYZERS ALLOWS THE USE OF COMMERCIALLY AVAILABLE ANTISERA AND REF SERA
IMMUNOTURBIDIMETRY
68
HYPERLIPOPROTEINEMIAA HAVE BEEN CLASSIFIED USING THE SYSTEM WHICH IS NKT COMMONLY USED TODAY
FREDRICKSON-LEVY CLASSIFICATION
69
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAS ELEVATED CHYLOMICRONS
TYPE I HYPERLIPOPROTEINEMIA
70
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAS INCREASED LDL
TYPE II HYPERLIPOPROTEINEMIA
71
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAD INCREASED LDL AND VLDL
TYPE IIB HYPERLIPOPROTEINEMIA
72
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAS INCREASED IDL
TYPE III HYPERLIPOPROTEINEMIA
73
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAS INCREASED VLDL
TYPE IV HYPERLIPOPROTEINEMIA
74
WHICH TYPE OF HYPERLIPOPROTEINEMIA HAS INCREASED VLDL AND CHYLOMICRONS
TYPE V HYPERLIPOPROTEINEMIA
75
4 TYPES OF HYPOLIPOPROTEINEMIAS
ABETALIPOPROTEINEMIA HYPOBETALIPOPROTEINEMIA HYPOALPHALIPOPROTEINEMIA TANGIER DISEASE
76
WHICH HYPOLIPOPROTEINEMIA IS TOTAL CHOLESTEROL IS VERY LOW AND TRIGLYCERIDE LEVEL IS UNDETECTABLE
ABETALIPOPROTEINEMIA
77
WHICH HYPOLIPOPROTEINEMIA IS UNABLE TO SYNTHESIZE APO B100 AND LOW CHOLESTEROL AND NORMAL TO LOW TAG
HYPOBETALIPOPROTEINEMIA
78
WHICH HYPOLIPOPROTEINEMIA IS SEVERELY ELEVATED TAG LEVEL AND LOW HDL LEVEL
HYPOALPHALIPOPROTEINEMIA
79
WHICH HYPOLIPOPROTEINEMIAS IS HDL ABSENT
TANGIER DISEASE

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