URIC ACID

Question 1

WHAT METHOD IS THIS:

URIC ACID IS OXIDIZED TO ______ AND _______ BY _______REDUCED TO______

Answer 1

DIRECT REDOX METHOD

ALLANTOIN AND CARBON DIOXIDE

BY PHOSPHOTUNGSTIC ACID REAGENT

TUNGSTEN BLUE

Question 2

WHAT METHOD IS THIS:

DIFFERENTIAL OR ABSORPTION SPECTROPHOTOMETRY

URIC ACID MAXIMUM ABSORPTION PEAK IS ______nm

URIC ACID IS STABLE FOR _________AT ______

_______ INCREASES ITS STABILITY TO ________

ANY ANTICOAGULANTS CAN BE USED EXCEPT ______ IT FORMS PHOSPHOTUNGSTATE PROMOTING ______
PURINE LIKE _____,_____ MAY AFFECT URIC ACID ASSAY

Answer 2

BLAUNCH OR KOCK METHOD (URICASE METHOD)

290-293 nm

SEVERAL DAYS AT ROOM TEMPERATURE

THYMOL; BACTERIAL DESTRUCTION

POTASSIUM OXALATE; TURBIDITY

LEGUMES, VISCERAL ORGAN

Question 3

WHAT METHOD IS THE OLDEST METHOD FOR URIC ACID DETERMINATION AND USES____AS COLOR STABILIZER

URIC ACID + PHOSPHOTUNGSTIC ACID=_____

Answer 3

CARAWAY METHOD

SODIUM CYANIDE

TUNGSTEN BLUE

Question 4

WHAT METHOD IS USED IN MODIFICATION OF CARAWAY AND USES ____ AS COLOR STABILIZER

Answer 4

HENRY’S METHOD

SODIUM CARBONATE

Question 5

PRINCIPLE OF URIC ACID DETERMINATION

Answer 5

URICASE

Question 6

DCHB STANDS FOR

Answer 6

3,5-DICHLORO-2-HYDROXYBENZENE-SULFONIC ACID

Question 7

END COLOR OF URIC ACID

Answer 7

RED VIOLET

Question 8

VALUE OF STANDARD OF URIC STANDARD

Answer 8

8mg/dl

Question 9

SPECIMEN USED IN URIC ACID DETER (3)

Answer 9

SERUM

PLASMA(EDTA/HEPARIN)

URINE (1:10 DILUTION WITH DISTILLED WATER

Question 10

SAMPLE STABILITY:

ROOM TEMPERATURE:
2-8C:
FROZEN/-20C:

Answer 10

2-3 DAYS

3-7 DAYS

6-12 MONTHS

Question 11

INTERFERENCE OF URIC ACID DETER (2)

Answer 11

HEMOGLOBIN >100mg/dl

BILIRUBIN >20mg/dl

Question 12

VOLUME OF URIC STANDARD AND SERUM

Answer 12

20UL OR 0.02mL

Question 13

INCUBATE AT ___ FOR ____

Or

ROOM TEMP FOR ______

Answer 13

37C FOR 5MINS

FOR 15 MINS

Question 14

WAVELENGTH USED IN URIC ACID DETERMINATION

Answer 14

520nm

Question 15

FORMULA USED IN URIC ACID DETERMINATION

Answer 15

BUA=UNK/STD X 8mg/dl FOR PLASMA/SERUM

BUA=UNK/STD X 80mg/dl FOR URINE

Question 16

NORMAL RANGE IN MALE

Answer 16

3.4-7.0mg/dl

Question 17

NORMAL RANGE IN FEMALE

Answer 17

2.4-5.7mg/dl

Question 18

LINEARITY LIMIT OF URIC ACID

Answer 18

0-20mg/dl

Question 19

NORMAL RANGE OF URINE SAMPLE

Answer 19

0.5-1.5g/day