Pseudomonas - Gutierrez

Question 1

Does psuedomonas form spores?

Answer 1

no

Question 2

Can pseudomonas spp ferment lactose?

Answer 2

No

Oxidase +

Question 3

Pseudomonas are _________ pathogens

Answer 3

Opportunistic

Question 4

What is the reservoir for pseudomonas?

Answer 4

Environment - water, soil, plants

Question 5

Pseudomonas aeruginosa is associated with what predisposing factors?

Answer 5

1. Trauma, burns, wounds
2. Malignancy
3. Immunodeficiency
4. Inbalance of microbiota

Question 6

True or False: Pseudomonas is involved in primary disease

Answer 6

False, secondary to other causes

Question 7

What is responsible for the blue- green color on culture of pseudomonas aeruginosa? What smell is associated?

Answer 7

Pyocyanin (siderophore), has a candy-sweet odor

Question 8

What virulence factors are on the cell surface of pseudomonas aeruginosa?

Answer 8

Flagella, pili, LPS

Question 9

What is responsible for the fluorescent green color on culture of pseudomonas aeruginosa?

Answer 9

Pyoverdin siderophore

Question 10

What is responsible for the red-brown color on culture of pseudomonas aeruginosa?

Answer 10

Pyorubin siderophore

Question 11

What is responsible for the brown/black color on culture of pseudomonas aeruginosa?

Answer 11

Pyomelanin siderophore

Question 12

How is pseudomonas aeruginosa transmitted?

Answer 12

Indirect contact through contaminated tools, water bowls, bedding, thermometers, etc

Question 13

What are the 3 stages involved in pseudomonas aeruginosa?

Answer 13

1. Adherence + colonization
2. Multiplication and growth (biofilm formation)
3. Systemic disease

Question 14

What toxins are involved in systemic pseudomonas aeruginosa disease?

Answer 14

LPS and EXOtoxin A

Question 15

What happens during growth and multiplication of pseudomonas aeruginosa?

Answer 15

bio film is formed, quorum sensing, T3SS is expressed

Question 16

How does pseudomonas aeruginosa adhere to host tissue?

Answer 16

Flagella and pili

Question 17

What are the major pathologies associated with pseudomonas aeruginosa in many species of animals?

Answer 17

1. Wound infections
2. UTI
3. Otitis externa + media
4. Resp tract infection

Question 18

pseudomonas aeruginosa can cause abortion in what species?

Answer 18

Horses and chinchillas

Question 19

What CS are associated with dogs and cats with pseudomonas aeruginosa?

Answer 19

1. Otitis externa - green pus, malodorous discharge
2. Lower UTI
3. Pneumonia
4. Pyoderma

Question 20

What CS are seen in horses with pseudomonas aeruginosa?

Answer 20

1. Infectious endometritis
2. Vaginitis
3. Abortion
4. Conjunctivitis

Question 21

What CS are seen in cows with pseudomonas aeruginosa?

Answer 21

Subclinical mastitis

Elevated somatic cell count in more chronic infections

Can have resp infection, uterine infection, enteritis

Question 22

What CS are seen in SHOATS with pseudomonas aeruginosa?

Answer 22

Gangrenous mastitis - high fatality rates

Fleece rot - superficial dermatitis, “green wool”

Question 23

Gangrenous mastitis is associated with what animal species and what bacteria?

Answer 23

SHOATS, pseudomonas aeruginosa

Question 24

What CS are seen in rabbits with pseudomonas aeruginosa?

Answer 24

1. Dermatitis
2. Pneumonia
3. Moist skin from automated water system

Question 25

The origin of pseudomonas aeruginosa comes from __________

Answer 25

contaminated drinking water

Question 26

What CS are seen in **mink** with pseudomonas aeruginosa?

Answer 26

1. Pulmonary lesions - pneumonia 2. High mortality

Question 27

What CS are seen in **chinchillas** with pseudomonas aeruginosa?

Answer 27

1. Abortion 2. Septicemia 3. Pneumonia 4. Otitis media 5. Sudden death

Question 28

What CS are seen in **reptiles** with pseudomonas aeruginosa??

Answer 28

1. Necrotic stomatits, "mouth rot" 2. Ulcers on skin 3. Pneumonia 4. Septicemia

Question 29

Reptiles are often infected with pseudomonas aeruginosa secondary to ___________

Answer 29

poor sanitary housing conditions

Question 30

What pseudomonas spp are fish most at risk for?

Answer 30

Pseudomonas fluorescens

Question 31

__________ is important in the spoilage of refrigerated milk, meat, eggs, seafood, and blood products

Answer 31

P. Fluorescens

Question 32

Rainbow trout with ulceration on the dorsal surface is associated with what bacteria spp?

Answer 32

Pseudomonas putida

Question 33

Who can be infected with pseudomonas fluorescens?

Answer 33

Fish - most pathogenic fish pathogen Poulty Cattle Humans (zoonotic)

Question 34

Which pseudomonas spp grow at 5 °C?

Answer 34

P. Fluorescens

Question 35

How can pseudomonas be prevented?

Answer 35

Prevent wet conditions Vaccine available only for minks

Question 36

Mouth rot is characteristic of ________ bacteria spp in ________ animals

Answer 36

Pseudomonas aeruginosa, reptiles

Question 37

P. fluorescens causes _______ in cattle

Answer 37

Mastitis

Question 38

P. fluorescens causes _______ in fish

Answer 38

Septicemia and tail/fin rot

Question 39

P. fluorescens causes _______ in poultry

Answer 39

Embryonic mortality

Question 40

Exophthalmia, ascites, and ulceration in tilapia is characteristic of ________ bacteria spp

Answer 40

Pseudomonas putida

Question 41

What does pseudomonas look like on macconkey agar?

Answer 41

Yellow bc does not ferment lactose - pale colonies with green-blue pyocyanin pigment

Question 42

True or False: Penicillin is used to treat pseudomonas

Answer 42

NOOOOO FALSE resistant to alot of broad spectrum antibiotics

Question 43

P. aeruginosa can cause necrotic stomatitis in ________

Answer 43

reptiles / snakes

Question 44

P. aeruginosa can cause dermatitis in __________

Answer 44

Sheep and rabbits

Question 45

P. aeruginosa can cause endocarditis in ____________

Answer 45

Dogs and cats