questions Flashcards
Describe how a sample of chloroplasts could be isolated from leaves (4 marks)
blend cells and filter
place in cold isotonic buffer
spin in centrifuge to remove nuclei
at higher speed chloroplasts settle out
Suggest one explanation for the faster rate of plasmid replication in cells growing in a culture with a high amino acid concentration (2 marks)
amino acids used in protein synthesis
more enzymes for DNA replication
Describe the role of one named organelle in digesting bacteria (3 marks)
lysosomes
fuse with vesicles/phagosome
releases lysozymes
Explain why it is not possible to determine the identity of the structures labelled X using an optical microscope (2 marks)
resolution too low
because light wavelength too long