quiz laboratorio #4 Flashcards
Many chemical reactions in biological systems have:
they are called:
high energy barriers that must be overcome before the reaction can proceed.
activation barriers
Between about ___________________ (physiological temperatures) most reactions…
10oC and 40oC
do not proceed without a catalyst.
Catalysts:
Hence, when an appropriate catalyst is provided, …
At the end of the reaction, the catalyst will…
accelerate reactions by lowering their activation energies
slow reactions can be made to occur rapidly.
remain unchanged from its original form.
Proteins called _______ function as catalysts, each one…
enzymes
designed to catalyze a specific type of reaction
. Enzymes are given names that
Enzymes provide organisms with a
reflect their specific functions, and typically end with the suffix “-ase.”
means to control which reactions occur at what time, and in which cell.
an appropriate enzyme must be…
In the exercises performed in this laboratory, some
presents at the proper location and it must be functional.
factors affecting the function of an enzyme shall be identified.
An enzyme-catalyzed reaction involves the interaction of the
The interaction occurs on the _______________ and produces an…
This complex produces at least ____________, ______________, while the enzyme then
-enzyme E with a reactant, or substrate S.
-enzyme’s active site
enzyme-substrate complex ES.
-one new molecule
the product P
then becomes available to interact with another substrate molecule.
objectives 4
-To understand enzyme-catalyzed reactions and the effects of pH and temperature on enzyme activity.
-To define catalyst, substrate, reactant, enzyme, denaturation, and hydrolysis.
-To use a spectrophotometric assay to construct a standard curve for the determination of starch concentration.
-To use a spectrophotometric assay to determine the time course of an enzyme-mediated reaction (starch hydrolysis by amylase)
-To investigate the pH and temperature dependence of starch hydrolysis by amylase.
