QUIZES COMPILATION

Question 1

The oil used in OIO for a clever view of the bacterial specimen

Answer 1

Cedar wood oil

Question 2

Primary stain used in simple staining

Answer 2

Methylene blue

Question 3

Scientist who introduced gram staining technique for studying microbes

Answer 3

Hans Christian Gram

Question 4

Counterstain used in gram staining

Answer 4

Safranin

Question 5

Acid fast was developed by ____ for the study of Mycobacterium spp.

Answer 5

Paul Ehrlich

Question 6

Primary stain used in acid fast staining technique

Answer 6

Carbolfuchsin

Question 7

Mordant used in gram staining

Answer 7

Iodine

Question 8

In gram staining method, gram positive organisms have high ________ in their cell walls whereas gram negative organisms have high proportion of ______

Answer 8

Peptidoglycan; Lipopolysaccharide

Question 9

Counterstain used in acid fast staining

Answer 9

Methylene blue

Question 10

Decolorizer used for acid fast staining

Answer 10

Acid alcohol

Question 11

Non-acid fast organisms are colored

Answer 11

Blue

Question 12

In the spore staining, what is the color of the endospores?

Answer 12

Green

Question 13

In capsule staining technique, what is the primary used for?

Answer 13

Crystal violet | Nigrosin | India Ink

Question 14

What stain was used in Negative staining

Answer 14

Nigrosin or congo red

Question 15

Plating method wherein a small sample volume is spread over the surface of an agar plate using a bent glass rod/tubing resulting in the formation of discrete colonies distributed evenly across the agar surface

Answer 15

Spread

Question 15

Plating method wherein a loopful of the diluted sample/inoculum is applied by streaking across the surface of the solidified media

Answer 15

Streak

Question 16

Plating method wherein bacteria are mixed with melted agar until evenly distributed throughout the media and poured into the plate

Answer 16

Pour plate method

Question 17

Four basic types of streaking method

Answer 17

• continous
• t-streak
• radiant
• quadrant

Question 18

Plates taken for bacterial counting should be in this range of colonies per plate

Answer 18

30-300

Question 19

Chemical which can bind with the cellular components and give them color

Answer 19

Stain

Question 20

Chemical agents that are used along with dye to make the specimen stainable, which otherwise is unstainable

Answer 20

Mordant

Question 21

Technique which is used to enhance and contrast a biological specimen at the microscopic level

Answer 21

Staining

Question 22

Passing the smear over low flame to kill microorganisms and fix them on the slide

Answer 22

Fixing

Question 23

Involves treating the specimen with a surfactant that dissolves the cell membrane allowing easy staining with the dye

Answer 23

Permeabilization

Question 24

Staining procedure used to identify bacteria based on their cell wall composition

Answer 24

Gram staining

Question 25

A three-color staining procedure which is used for differentiating cells from connective tissues

Answer 25

Masson staining

Question 26

Primary stain used in gram staining

Answer 26

Crystal violet

Question 27

Decolorizer used in gram’s staining

Answer 27

Ethanol

Question 28

Counterstain used in gram staining

Answer 28

Safranin

Question 29

Staining technique that uses only one type of stain on a slide at a time

Answer 29

Simple staining

Question 30

A gelatinous outer layer secreted by bacterial cell and that surrounds and adheres to the cell wall

Answer 30

Capsule

Question 31

These are metabolic inactive, highly resistant structures produced by some bacteria as a defensive strategy against unfavorable environmental conditions

Answer 31

Endospores

Question 32

Staining technique that colors the background but not the organism being studied

Answer 32

Negative staining

Question 33

A mixture of substances that promotes and supports the growth and differentiation of microorganisms in the laboratory

Answer 33

Culture

Question 34

Media that allows the growth of certain microbes or desired microorganisms and inhibits the growth of unwanted microbes

Answer 34

Selective media

Question 35

Media that shows visible changes due to the presence of an indicator like dyes or metabolic substances in the medium which gives different colors to the colonies of different microbial species when they utilize or react with these components

Answer 35

Differential media