ON XLD: 1. COLORLESS COLONIES 2. RED COLONIES WITH BLACK CENTERS 3. YELLOW COLONIES

1. Shigella 2. Salmonella 3. [EKE]

RECALLS 💡 Flashcards by J .

modified Acid Fast stain DECOLORIZER

ACID ALCOHOL

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done to recover and identify Clostridium spp from mixed populations of organisms

ETHANOL SHOCK TEST

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Mycobacterium spp that can cause pulmonary infection RESEMBLING M. tuberculosis

Mycobacterium xenopi

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often recovered from HOT WATER TAPS & CONTAMINATED WATER SYSTEMS

Mycobacterium xenopi

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used for the detection of LIPASE and LECITHINASE PRODUCTION

EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR

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(+) opaque precipitate within agar or IRIDESCENT SHEEN or PEARLY LAYER on the surface of the agar

EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR

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primary PHYSICAL barrier

SKIN

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contact time of IODINE ON SKIN FOR BLOOD CULTURE

60 SECONDS

or 30 seconds pero mas preferable ang 60 seconds

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spirochete OFTEN DETECTED IN HEMA LAB

Borrelia

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method of choice for DIAGNOSIS OF LYME DISEASE

SEROLOGIC TEST (IF or EIA)

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U agar with Urea and Phenol Red is for what organism?

Ureaplasma urealyticum

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medium of choice for theSELECTIVE RECOVERY OF GRAM (-) ANAEROBES

KANAMYCIN-VANCOMYCIN LAKE BLOOD (KVLB)

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Mycobacterium isolated FROM SUBCUTANEOUS SKIN (+) GROWTH at 30 degC

Mycobacterium marinum
Mycobacterium ulcerans
Mycobacterium haemophilum

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ON XLD:

COLORLESS COLONIES
RED COLONIES WITH BLACK CENTERS
YELLOW COLONIES

Shigella
Salmonella
[EKE]

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common laboratory acquired infections

HEPATITIS
SALMONELLOSIS
TB
SHIGELLOSIS
BRUCELLOSIS

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MOST IMPORTANT practice to prevent spread of infection

HANDWASHING

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most susceptible to infection

WARM MOIST SKIN

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MOST COMMON method of TREATING INFECTIOUS WASTE

INCINERATION

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CSF and blood should always be kept at what temperature?

35-37°C

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variables that may change the MIC

INOCULUM SIZE
INCUBATION TIME
GROWTH RATE OF BACTERIA

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members of Enterobacteriaceae that MOST COMMONLY PRODUCE ESBL

E. coli
K. pneumoniae

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the ONLY NON-FERMENTER that produces ABUNDANT H2S on KIA or TSI

Shewanella putrefaciens

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M agar with Arginine and Phenol Red is for what bacteria?

Mycoplasma

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M. tuberculosis cultures are held in the lab after identification and AST for how long?

6 MONTHS

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**BACKUP BROTH** is also called

SUPPLEMENTAL BROTH

**LIM BROTH** is for what organism?

*S. agalactiae*

what DOH memorandum number requires **2 SPX IN 1 DAY FOR TB DETECTION**?

DOH MEMORANDUM NO. **2023-0021**

In DOH Memo No. 2023-0021, how many sputum specimens are to be collected for TB detection?

2 SPX IN 1 DAY

plan that identifies tasks that are hazardous to employees and promote employee safety

EXPOSURE CONTROL PLAN

the Exposure Control Plan is the responsibility of who?

MICROBIOLOGY SUPERVISOR & LAB DIRECTOR (CMT)

**flexible calcium alginates nasopharyngeal swab** is the device of choice for...?

*Corynebacterium diphtheriae*

CAP can provice X and V factor for...?

Haemophilus

CCFA with neutral red will make colonies of *C. difficile* what color?

YELLOW | because of **FRUCTOSE FERMENTATION**

VP test detects which product of glucose fermentation?

ACETOIN or ACETYL METHYL CARBINOL

amine product of **ORNITHINE DECARBOXYLATION**

PUTRESCINE

organisms that may produce **YELLOW SLANT ON TSI but RED SLANT ON KIA**

1. Citrobacter 2. Serratia 3. Edwardsiella

**MOST COMMONLY IMPLICATED** in **DIARRHEAL DISEASE**

1. Escherichia 2. Salmonella 3. Shigella 4. Yersinia

produces **WRINKLED COLONIES** but differentiated thru biochemical tests

1. *Pseudomonas stutzeri* 2. *Burkholderia pseudomallei*

**CETRIMIDE AGAR** is for the ID of what organism?

*Pseudomonas aeruginosa*

**CETRIMIDE AGAR** is aka...

ACETYL TRIMETHYL AMMONIUM BROMIDE AGAR

Pyocyanin (blue pigment) of *P. aeruginosa* can be noted on what medium?

MHA

what is used to differentiate **NON-PATHOGENIC Moraxella** **growing on selected media for Neisseria** from Neisseria species?

10 UNITS OF PENICILLIN DISKS

Gram (+) slender rods **GROWING ON TOMATO JUICE AGAR**

Lactobacillus

**MANGANOUS CHLORIDE UREA TEST** is rapid test for what organism?

*U. urealyticum*

resistance of *N. gonorrhoeae* to Penicillin is due to

BETA LACTAMASE

collection of urine from px with **INDWELLING CATHETER**

ASPIRATE ASEPTICALLY FROM THE CATHETER TUBING

AST method **RECOMMENDED TO DETECT VANCOMYCIN INTERMEDIATE *S. aureus***

MIC BROTH DILUTION METHOD

effect of placing antibiotic disk after organism inoculation and incubating within 15 minutes after disk placement

DECREASES ZONE DIAMETER

**MISTAKEN FOR *N. gonorrhoeae*** in gram-stained smears from UTERINE CERVIX EXUDATE

*Moraxella osloensis*

antimicrobial combination useful in **CONFIRMING PRESENCE OF ESBL IN *E. coli***

CEFTAZIDIME nad CLAVULANIC ACID

yeast enzyme detected by **BIRDSEED AGAR**

PHENOL OXIDASE

**DO NOT FORM PSEUDOHYPHAE OR ARTHROCONIDIA**; produces only Blastoconidia

Cryptococcus

produces Pseudohyphae and Blastoconidia; will **NOT PRODUCE ARTHROCONIDIA**

Candida

produces **PSEUDOHYPHAE, BLASTOCONIDIA, and ATHROCONIDIA**

Trichosporon

produce **PSEUDOHYPHAE** and **ARTHROCONIDIA**

Geotrichum

produce **ASCOSPORES**

*Saccharomyces cerevisiae*

a rare cause of Cat Scratch Disease

AFIPIA FELIS

test of choice for the **CONFIRMATION OF *C. trachomatis* ** INFECTION IN URINE

PCR MOLECULAR TESTING

the **MOST RELIABLE** for the detection of *Mycoplasma pneumoniae* in serum and for the **CONFIRMATION OF DIAGNOSIS**

EIA and DIRECT ANTIGEN TESTING

features in which **MOLDS** are routinely categorized

1. MACROSCOPIC GROWTH CHARACTERISTICS 2. MICROSCOPIC MORPHOLOGY

**MOST SENSITIVE TEST** for the initial dx of cryptococcal disease

CRYPTOCOCCAL ANTIGEN

microscopic structures that are useful in the identification of **DERMATOPHYTES**

1. MACROCONIDIA 2. MICROCONIDIA

most useful in distinguishing yeast morphology

CORN MEAL AGAR WITH TWEEN 80

encapsulated fungus associated with Eucalyptus tree

*Cryptococcus gattii*

spx of choice for **RSV DETECTION**

NASOPHARYNGEAL ASPIRATE

**MOST COMMON ANAEROBIC BACTERIA ** isolated from infectious processes of **SOFT TISSUES and BACTEREMIA**

*Bacteroides fragilis*

**MOST SENSITIVE** for the recovery of Brucella in CHRONIC INFECTION

BONE MARROW

**ACCEPTABLE** anticoagulants for Blood, BM, and Synovial Fluid that are to be cultured

1. SPS 2. HEPARIN

**UNIVERSALLY SUSCEPTIBLE TO PENICILLIN**

*S. pyogenes*

primary marker of **FECAL CONTAMINATION**

*E. coli*